REL 2022-11-10 2023-02-03 2023-06-16 Cryo-electron tomography on plasma FIB lamellae of HeLa cells 0000-0003-2580-2299 Michael Grange Rosalind Franklin Institute Harwell campus Oxfordshire United Kingdom OX11 0QX 0000-0003-2580-2299 Michael Grange Rosalind Franklin Institute Harwell campus Oxfordshire United Kingdom OX11 0QX Berger C Dumoux M Glen T Yee NB Mitchels JM Patáková Z Darrow MC Naismith JH Grange M 622.2 10.6019/EMPIAR-11306 EMDB cell EMD-15636 Berger C Dumoux M Glen T Yee NB Mitchels JM Patáková Z Darrow MC Naismith JH Grange M Plasma FIB milling for the determination of structures in situ Nature communications Nat Commun 1 14 2023 English 10.1038/s41467-023-36372-9 36746945
180 tilt-series acquired on argon plasma FIB lamellae with a Titan Krios G4 equipped with a Selectris energy filter and Falcon 4 camera recorded in EER format. Data was used to obtain a 4.9 Å structure of the human ribosome.
 Tilt-series recorded on argon PFIB lamellae of HeLa cells /data/HeLa_argon tilt series TIFF EER 6481 10 4 BIT INTEGER 4096 1.9 4096 1.9
Tilt-series recorded on argon PFIB lamellae of HeLa cells using a dose-symmetric tilt-scheme corrected for the pre-tilt of the lamellae. Electron dose: 5 e/A2 per tilt. Data recorded at a pixel size of 1.85 A/px, which was refined to 1.9A/px based on the Subtomogram average structure.