REL
2022-11-04
2022-11-15
2022-11-15
Reconstituted USP1, UAF1, FANCI, mono-ubiquitinated FANCD2 and the inhibitor ML323
0000-0002-0799-3450
Martin
Luke
Rennie
School of Molecular Biosciences, College of Medical, Veterinary & Life Sciences, University of Glasgow
University Ave
Glasgow
Lanarkshire
United Kingdom
G128QQ
0000-0002-4289-4810
Helen
Walden
School of Molecular Biosciences, College of Medical, Veterinary & Life Sciences, University of Glasgow
University Ave
Glasgow
Lanarkshire
United Kingdom
G12 8QQ
Rennie ML
Walden H
2.3
10.6019/EMPIAR-11299
EMDB
molecule
EMD-15284
EMD-14722
EMD-14720
EMD-14721
EMD-14719
Rennie ML
Arkinson C
Chaugule VK
Walden H
Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site
Science advances
Sci Adv
39
8
eabq6353
N/A
2022
English
10.1126/sciadv.abq6353
36170365
Raw movie frames without alignment, without gain correction. Accumulating 40 electrons per squared Angstrom over 40 frames at a rate of 15.46 electrons per pixel per second)
Unaligned multi-frame micrographs of reconstituted USP1, UAF1, FANCI, monoubiquitinated FANCD2, DNA, and ML323
/data
micrographs - multiframe
TIFF
TIFF
10998
40
UNSIGNED BYTE
4092
1.06
5760
1.06
Each multi-frame raw micrograph is composed of 40 frames each in compressed TIFF format, along with a separate gain reference. Multi-frame micrographs were collected at 81,000x nominal magnification at 15.46 e-/pix/s in super-resolution mode with 2x binning on a Gatan K3. Data collected on KriosIV at eBIC on 2021-11-29.
Sample contained 9.3 μM USP1, 9.3 μM UAF1, 1.8 μM FANCI, 1.8 μM FANCD2 monoubiquitinated on K561, 2.2 μM dsDNA (61 base pairs), and 18.3 μM ML323.
data/data_*/*.tiff