REL 2022-08-02 2023-04-12 2023-04-13 0000-0001-6768-3406 Stephen G Brohawn UC Berkeley Weill Hall Berkeley California United States 94720 0000-0001-8529-9045 David M Kern UC Berkeley Weill Hall Berkeley CA United States 94720 0000-0001-6768-3406 Stephen G Brohawn UC Berkeley Weill Hall Berkeley California United States 94720 Kern DM Brohawn SG 8.9 10.6019/EMPIAR-11283 EMDB molecule EMD-27682 EMD-27677 EMD-27678 EMD-27679 EMD-27681 EMD-27676 EMD-27674 EMD-27675 Kern DM Bleier J Mukherjee S Hill JM Kossiakoff AA Isacoff EY Brohawn SG bioRxiv 2022 English 10.1101/2022.07.31.502239 30775971 Unaligned tif movies for LRRC8ABRIL:C in 150 mM KCl /data/frames_150 micrographs - multiframe TIFF TIFF 8261 50 UNSIGNED BYTE 11520 0.524 8184 0.524

Gain reference in dm4 and mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 1.076 e/Å2 dose per frame and use bin 2 to produce mrc at the physical pixel size of 1.048Å/px.

Unaligned tif movies for LRRC8ABRIL:C in 75 mM KCl /data/frames_75 micrographs - multiframe TIFF TIFF 7208 50 UNSIGNED BYTE 11520 0.524 8184 0.524

Gain reference in dm4 and mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 0.9953 e/Å2 dose per frame and use bin 2 to produce mrc at the physical pixel size of 1.048Å/px.

Pooled polished particles stacks for LRRC8A-BRIL/C in GDN /data/PolishedParticles picked particles - multiframe - processed MRCS MRCS 11609 1 UNSIGNED BYTE 416 1.048 416 1.048

Contains polished particles (437,174) as 11,609 mrcs files from the pooled GDN samples (75 and 150 mM KCl). The selections of particles for each conformation (1 and 2) are in the main data folder. They are in RELION star format and can be imported into cryoSPARC as well for processing.