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            <depositionDate>2022-10-18</depositionDate>
            <releaseDate>2022-11-11</releaseDate>
            <updateDate>2023-01-12</updateDate>
        </keyDates>
        <title>Cryo-electron tomography of microtubules assembled in Xenopus egg cytoplasmic extracts</title>
        <correspondingAuthor>
            <authorORCID>0000-0001-8261-4396</authorORCID>
            <firstName>Denis</firstName>
            <lastName>Chrétien</lastName>
            <organization type="academic">Institute of Genetics and Development of Rennes</organization>
            <street>263 avenue du Général Leclerc, Bâtiment 13</street>
            <townOrCity>Rennes</townOrCity>
            <country>France</country>
            <postOrZipCode>35042</postOrZipCode>
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            <firstName>Denis</firstName>
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            <street>263 avenue du Général Leclerc, Bâtiment 13</street>
            <townOrCity>Rennes</townOrCity>
            <country>France</country>
            <postOrZipCode>35042</postOrZipCode>
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        <authorsList>
            <author authorORCID="0000-0001-9766-3932">Guyomar C</author>
            <author authorORCID="0000-0002-6905-0386">Bousquet C</author>
            <author>Ku S</author>
            <author authorORCID="0000-0001-6751-3028">Heumann J</author>
            <author authorORCID="0000-0003-4674-3478">Guilloux G</author>
            <author authorORCID="0000-0002-1129-1291">Gaillard N</author>
            <author>Heichette C</author>
            <author authorORCID="0000-0003-1985-6266">Duchesne L</author>
            <author authorORCID="0000-0001-6157-3687">Steinmetz MO</author>
            <author authorORCID="0000-0001-5081-1985">Gibeaux R</author>
            <author authorORCID="0000-0001-8261-4396">Chrétien D</author>
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        <scale>molecule</scale>
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                    <author authorORCID="0000-0001-9766-3932" order="1">Guyomar C</author>
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                    <author order="3">Ku S</author>
                    <author order="4">Heumann J</author>
                    <author authorORCID="0000-0003-4674-3478" order="5">Guilloux G</author>
                    <author authorORCID="0000-0002-1129-1291" order="6">Gaillard N</author>
                    <author order="7">Heichette C</author>
                    <author authorORCID="0000-0003-1985-6266" order="8">Duchesne L</author>
                    <author authorORCID="0000-0001-6157-3687" order="9">Steinmetz MO</author>
                    <author authorORCID="0000-0001-5081-1985" order="10">Gibeaux R</author>
                    <author authorORCID="0000-0001-8261-4396" order="11">Chrétien D</author>
                    <title>Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in &lt;i&gt;Xenopus&lt;/i&gt; egg cytoplasmic extracts</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <year>2021</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1101/2021.07.14.452321</externalReferences>
                    <details>Dual-axis tilt series of microtubules assembled in Xenopus egg cytoplasmic extracts, decorated with kinesin motor domains, and vitrified by plunge freezing into liquid ethane</details>
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                    <author order="1">Guyomar C</author>
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                    <author order="4">Heumann JM</author>
                    <author order="5">Guilloux G</author>
                    <author order="6">Gaillard N</author>
                    <author order="7">Heichette C</author>
                    <author order="8">Duchesne L</author>
                    <author order="9">Steinmetz MO</author>
                    <author authorORCID="0000-0001-5081-1985" order="10">Gibeaux R</author>
                    <author authorORCID="0000-0001-8261-4396" order="11">Chrétien D</author>
                    <editor order="1"> </editor>
                    <title>Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in &lt;i&gt;Xenopus&lt;/i&gt; egg cytoplasmic extracts</title>
                    <journal>eLife</journal>
                    <journalAbbreviation>Elife</journalAbbreviation>
                    <country></country>
                    <volume>11</volume>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.7554/elife.83021</externalReferences>
                    <externalReferences type="pubmed">36503602</externalReferences>
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    <imageSet>
        <name>Dual-axis tilt series used to perform segmented sub-tomogram averaging of microtubules assembled in Xenopus egg cytoplasmic extracts and decorated with kinesin motor domains</name>
        <directory>/data/raw_tilt_series</directory>
        <category>tilt series</category>
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        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>6</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
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            <imageWidth>2048</imageWidth>
            <pixelWidth>7.5</pixelWidth>
            <imageHeight>2048</imageHeight>
            <pixelHeight>7.5</pixelHeight>
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        <details>Dual-axis tilt series, the number of images is variable. The pixel spacing of Xen_D_tomo1a and Xen_D_tomo1b is 8.9 Å.</details>
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        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
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