Structure of an Escherichia coli 70S ribosome stalled by Tetracenomycin X during translation of an MAAAPQK(C) peptide

REL 2022-09-07 2023-07-10 2023-07-10 Structure of an Escherichia coli 70S ribosome stalled by Tetracenomycin X during translation of an MAAAPQK(C) peptide 0000-0003-3153-9490 C Axel Innis INSERM U1212 CNRS UMR 5320 University of Bordeaux 146 rue Léo Saignat Bordeaux Nouvelle-Aquitaine France 33076 0000-0003-3153-9490 C Axel Innis INSERM U1212 CNRS UMR 5320 University of Bordeaux 146 rue Léo Saignat Bordeaux Nouvelle-Aquitaine France 33076 0000-0002-1530-2613 Thibaud Renault INSERM U1212 CNRS UMR 5320 University of Bordeaux 146 rue Léo Saignat Bordeaux Nouvelle-Aquitaine France 33076 Leroy EC Perry TN Renault TT Innis CA 948.5 10.6019/EMPIAR-11241 EMDB molecule EMD-14956 Leroy EC Perry TN Renault TT Innis CA Tetracenomycin X sequesters peptidyl-tRNA during translation of QK motifs Nature chemical biology Nat Chem Biol 2023 English 10.1038/s41589-023-01343-0 37322159

The micrographs were recorded on a Talos Arctica (Thermo Fisher Scientific) operated at 200 kV and equipped with a K2 Summit direct electron detector (Gatan) in Nanoprobe mode. SerialEM was used with a magnified pixel size of 0.93 Å at a magnification of 45,000x to record 38 movie frames with an exposure time of 3.8 seconds using a dose rate of 0.94 electrons/Å² per frame for a total accumulated dose of 35.72 electrons/Å².

Unaligned multi-frame micrographs of an E. coli 70S-MAAAPQKC-TcmX ribosomal complex /data/frames micrographs - multiframe TIFF TIFF 6156 38 1 38 UNSIGNED 16 BIT INTEGER 3710 0.905 3838 0.905

Raw micrographs collected on a Talos Arctica with a nominal pixsize of 0.93Å/px (calibrated 0.905Å/px) of an E. coli 70S-MAAAPQKC-TcmX ribosomal complex.

Gain reference /data/gain micrographs - single frame TIFF TIFF 1 1 UNSIGNED 16 BIT INTEGER 3710 0.905 3838 0.905

Gain must be rotated 270°.