REL 2022-09-16 2022-11-16 2022-11-16 0000-0002-9901-2065 Jan Rheinberger Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG 0000-0001-7017-109X Cristina Paulino Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG Silberberg JM Stock C Hielkema L Corey RA Rheinberger J Wunnicke D Dubach VRA Stansfeld PJ Hänelt I Paulino C 2.5 10.6019/EMPIAR-11229 EMDB molecule EMD-14914 EMD-14915 EMD-14916 Silberberg JM Stock C Hielkema L Corey RA Rheinberger J Wunnicke D Dubach VRA Stansfeld PJ Hänelt I Paulino C eLife Elife 11 2022 English 10.7554/elife.80988 36255052 Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 1) /data/raw_181029_KdpFABC-B-D307N-KCl-bCP75s3_ngc micrographs - multiframe TIFF TIFF 787 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 2) /data/raw_181031_KdpFABC-B-D307N-KCl-bCP75s5_ngc micrographs - multiframe TIFF TIFF 1370 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 3) /data/raw_181205_KdpFABC-B-D307N-KCl-bCP75s2_ngc micrographs - multiframe TIFF TIFF 2213 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 4) /data/raw_181214_KdpFABC-B-D307N-KCl-bCP75s6_ngc micrographs - multiframe TIFF TIFF 1333 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 5) /data/raw_190320_KdpFABC-B-D307N-KCl-bLH18s7_ngc micrographs - multiframe TIFF TIFF 317 60 UNSIGNED BYTE 3838 1.012 3838 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 6) /data/raw_190322_KdpFABC-B-D307N-KCl-bLH18s6_ngc micrographs - multiframe TIFF TIFF 618 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 7) /data/raw_190403_KdpFABC-B-D307N-KCl-bLH18s3_ngc micrographs - multiframe TIFF TIFF 181 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 8) /data/raw_190404_KdpFABC-B-D307N-KCl-bLH19s2_ngc micrographs - multiframe TIFF TIFF 448 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 9) /data/raw_190613_KdpFABC-B-D307N-KCl-bLH21s3_ngc micrographs - multiframe TIFF TIFF 93 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 10) /data/raw_190614_KdpFABC-B-D307N-KCl-bLH21s7_ngc micrographs - multiframe TIFF TIFF 959 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 11) /data/raw_190626_KdpFABC-B-D307N-KCl-bLH21s2_ngc micrographs - multiframe TIFF TIFF 862 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 12) /data/raw_190807_KdpFABC-B-D307N-KCl-bLH22s1_ngc micrographs - multiframe TIFF TIFF 897 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 13) /data/raw_190809_KdpFABC-B-D307N-KCl-bLH22s2_ngc micrographs - multiframe TIFF TIFF 1248 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 14) /data/raw_190814_KdpFABC-B-D307N-KCl-bLH23s1_ngc micrographs - multiframe TIFF TIFF 584 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 15) /data/raw_190816_KdpFABC-B-D307N-KCl-bLH23s2_ngc micrographs - multiframe TIFF TIFF 954 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.