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        <keyDates>
            <depositionDate>2022-09-19</depositionDate>
            <releaseDate>2023-02-01</releaseDate>
            <updateDate>2023-02-01</updateDate>
        </keyDates>
        <title>CryoET tilt series of mouse sperm flagella after FIB-SEM milling</title>
        <correspondingAuthor>
            <authorORCID>0000-0003-1723-0251</authorORCID>
            <firstName>Zhen</firstName>
            <lastName>Chen</lastName>
            <organization type="academic">UCSF</organization>
            <street>600 16th St</street>
            <townOrCity>SAN FRANCISCO</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94158</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <firstName>Ronald</firstName>
            <lastName>Vale</lastName>
            <organization type="academic">Janelia Research Campus</organization>
            <street>19700 Helix Dr</street>
            <townOrCity>Ashburn</townOrCity>
            <stateOrProvince>VA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>20147</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-1723-0251">Chen Z</author>
            <author>Greenan GA</author>
            <author>Shiozaki M</author>
            <author>Liu Y</author>
            <author>Skinner WM</author>
            <author>Zhao X</author>
            <author>Zhao S</author>
            <author>Yan R</author>
            <author>Guo C</author>
            <author>Yu Z</author>
            <author>Lishko PV</author>
            <author>Agard DA</author>
            <author>Vale RD</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
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        <datasetSize>194.0</datasetSize>
        <entryDOI>10.6019/EMPIAR-11221</entryDOI>
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        <scale>cell</scale>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-27444</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-1723-0251" order="1">Chen Z</author>
                    <author order="2">Greenan GA</author>
                    <author order="3">Shiozaki M</author>
                    <author order="4">Liu Y</author>
                    <author authorORCID="0000-0002-2383-4003" order="5">Skinner WM</author>
                    <author order="6">Zhao X</author>
                    <author order="7">Zhao S</author>
                    <author order="8">Yan R</author>
                    <author authorORCID="0000-0002-7114-2570" order="9">Yu Z</author>
                    <author authorORCID="0000-0003-3140-2769" order="10">Lishko PV</author>
                    <author authorORCID="0000-0003-3512-695X" order="11">Agard DA</author>
                    <author authorORCID="0000-0003-3460-2758" order="12">Vale RD</author>
                    <title>In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes</title>
                    <journal>Nature structural &amp; molecular biology</journal>
                    <journalAbbreviation>Nat Struct Mol Biol</journalAbbreviation>
                    <country></country>
                    <year>2023</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41594-022-00861-0</externalReferences>
                    <externalReferences type="pubmed">36593309</externalReferences>
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            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0003-1723-0251" order="1">Chen Z</author>
                    <author order="2">Greenan GA</author>
                    <author order="3">Shiozaki M</author>
                    <author order="4">Liu Y</author>
                    <author order="5">Skinner WM</author>
                    <author order="6">Zhao X</author>
                    <author order="7">Zhao S</author>
                    <author order="8">Yan R</author>
                    <author order="9">Guo C</author>
                    <author order="10">Yu Z</author>
                    <author order="11">Lishko PV</author>
                    <author order="12">Agard DA</author>
                    <author order="13">Vale RD</author>
                    <title>&lt;i&gt;In situ&lt;/i&gt; cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1101/2022.05.15.492011</externalReferences>
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    <imageSet>
        <name>Tilt series and tilt angle files</name>
        <directory>/data</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>69</numImagesOrTiltSeries>
        <framesPerImage>37</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>37</frameRangeMax>
        </frameRange>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>4092</imageWidth>
            <pixelWidth>3.53</pixelWidth>
            <imageHeight>5760</imageHeight>
            <pixelHeight>3.53</pixelHeight>
        </dimensions>
        <details>There are 69 tilt series with 69 tilt angle files. The number of tilt images varied because some high tilt images are blocked by ice or lost due to tracking issues.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
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