REL
2022-03-23
2022-08-16
2022-08-18
Cryo-EM structure of the human GS-GN complex in the inhibited state
0000-0003-0239-5926
Elton
Zeqiraj
Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
Leeds
United Kingdom
LS2 9JT
0000-0003-0239-5926
Elton
Zeqiraj
Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
Leeds
United Kingdom
LS2 9JT
Marr L
Biswas D
Daly LA
Browning C
Vial SCM
Maskell DP
Hudson C
Bertrand JA
Pollard J
Ranson NA
Khatter H
Eyers CE
Sakamoto K
Zeqiraj E
9.2
10.6019/EMPIAR-11139
EMDB
molecule
EMD-14587
Marr L
Biswas D
Daly LA
Browning C
Vial SCM
Maskell DP
Hudson C
Bertrand JA
Pollard J
Ranson NA
Khatter H
Eyers CE
Sakamoto K
Zeqiraj E
Mechanism of glycogen synthase inactivation and interaction with glycogenin
Nature communications
Nat Commun
1
13
2022
English
10.1038/s41467-022-31109-6
35690592
Unaligned multiframe movies in the EER format of the human glycogen synthase-glycogenin complex
/data/Images-Disc1
micrographs - multiframe
EER
EER
20841
896
BIT
variable
0.71
variable
0.71
A dose per physical pixel/s of 4.71 was used resulting in a total dose of 34.8 e/Å2, fractionated across 128 EPU frames. This was then grouped into 21 frames, resulting in a dose per frame of 0.8 e/Å2. Micrograph file pattern: data/Images-Disc1/GridSquare_*/Data/*.eer
Aligned micrographs of the human glycogen synthase-glycogenin complex
/data/job008
micrographs - single frame
MRC
MRC
20841
1
32 BIT FLOAT
4096
0.71
4096
0.71
Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 21. Micrograph file pattern: data/job008/Raw_data/*.mrc