REL 2022-06-30 2022-09-12 2022-09-12 Structures of the Cyanobacterial Phycobilisome in the Light-harvesting and Photoprotected States 0000-0001-7204-5863 Paul Victor Sauer QB3 Institute, University of California, Berkeley, CA, USA 742 Stanley Hall Berkeley CA United States 94720 0000-0002-9977-8482 Cheryl A Kerfeld MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA East Lansing MI United States 48824 Sauer PV Dominguez-Martin MA Kerfeld CA 16.7 10.6019/EMPIAR-11133 EMDB molecule EMD-25029 EMD-25028 EMD-25069 EMD-25070 EMD-25071 EMD-25030 EMD-25031 EMD-25032 EMD-25033 EMD-25068 Domínguez-Martín MA Sauer PV Kirst H Sutter M Bína D Greber BJ Nogales E Polívka T Kerfeld CA Structures of a phycobilisome in light-harvesting and photoprotected states Nature Nature 2022 English 10.1038/s41586-022-05156-4 36045294
Datasets 1(PBS-apo), 2 (PBS-apo) and 3 (OCP-PBS) from the paper; This entry contains the motion-corrected and lattice-subtracted micrographs and the raw movie files. This entry also contains Cryosparc particle files and passthrough files (.cs format) for each reconstruction from datasets 2 and 3.
 Dataset 3: PBS-OCP micrographs motion corrected and streptavidin subtracted /data/Frames_sub_correctdose micrographs - single frame MRC MRC 13427 1 32 BIT FLOAT 5760 1.05 4092 1.05
Data collected on 300kV Krios G3 with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.
 Dataset 2: PBS-apo micrographs motion corrected and streptavidin subtracted /data/Frames_sub_20Dec21 micrographs - single frame MRC MRC 12051 1 32 BIT FLOAT 5760 1.05 4092 1.05
Data collected on 300kV Krios G3 with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.
 Dataset 1: PBS-apo micrographs motion corrected and streptavidin subtracted /data/Frames_grid3_sub micrographs - single frame MRC MRC 1954 1 32 BIT FLOAT 5760 1.115 4092 1.115
Data collected on 200kV Arctica with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.
 Dataset 3: PBS-OCP raw movies unaligned and unsubtracted /data/Frames_dataset1 micrographs - multiframe TIFF TIFF 13452 50 UNSIGNED BYTE 11520 0.524 8184 0.524
Data collected on 300kV Krios G3 with K3 detector. Total dose of 50e/A2. Contains streptavidin signal. Gain reference in .dm4 format.
 Dataset 2: PBS-apo raw movies unaligned and unsubtracted /data/Frames_dataset2 micrographs - multiframe TIFF TIFF 12387 50 UNSIGNED BYTE 11520 0.524 8184 0.524
Data collected on 300kV Krios G3 with K3 detector. Total dose of 50e/A2. Contains streptavidin signal. Gain reference in .dm4 format.