REL 2022-07-25 2022-09-13 2022-11-11 0000-0001-7250-4484 Gregory M. Alushin Laboratory of Structural Biophysics and Mechanobiology, The Rockefeller University New York NY United States 10065 0000-0001-7250-4484 Gregory M. Alushin Laboratory of Structural Biophysics and Mechanobiology, The Rockefeller University New York NY United States 10065 Reynolds MJ Hachicho C Carl AG Gong R Alushin GM 938.5 10.6019/EMPIAR-11128 EMDB molecule EMD-27114 EMD-27116 EMD-27118 EMD-27119 Reynolds MJ Hachicho C Carl AG Gong R Alushin GM Nature Nature 7935 611 380 386 2022 English 10.1038/s41586-022-05366-w 36289330

This dataset was used to generate reconstructions of helically-symmetric ADP-F-actin, bent ADP-F-actin, and straight asymmetrically reconstructed ADP-F-actin.

Unaligned multi-frame micrographs of ADP-F-actin /data/raw_micrographs micrographs - multiframe TIFF TIFF 1548 40 UNSIGNED BYTE 7420 0.515 7676 0.515

This directory also contains motion-correction that was done on-the-fly. Re-running motion-correction locally is recommended.

Polished single-frame particles of bent ADP-F-actin segments /data/ADP_asymmetric_particle_stacks/bent picked particles - single frame - processed MRCS MRCS 10753 1 32 BIT FLOAT 512 1.03 512 1.03

This stack of particles was generated from the final Refine3D of the asymmetric, bent ADP-F-actin at EMD-27116 and PDB: 8D15 using the relion_stack_create command.

Polished single-frame particles of straight ADP-F-actin segments, Subset 1 /data/ADP_asymmetric_particle_stacks/str1 picked particles - single frame - processed MRCS MRCS 7833 1 32 BIT FLOAT 512 1.03 512 1.03

This stack of particles was generated from the final Refine3D of the asymmetric, straight ADP-F-actin at EMD-27118 and PDB: 8D17 using the relion_stack_create command.

Polished single-frame particles of straight ADP-F-actin segments, Subset 2 /data/ADP_asymmetric_particle_stacks/str2 picked particles - single frame - processed MRCS MRCS 7833 1 32 BIT FLOAT 512 1.03 512 1.03

This stack of particles was generated from the final Refine3D of the asymmetric, straight ADP-F-actin at EMD-27119 and PDB: 8D18 using the relion_stack_create command.

Polished single-frame particles of helical ADP-F-actin segments, for high-resolution /data/helical_polished_stack picked particles - single frame - processed MRCS MRCS 219569 1 32 BIT FLOAT 512 1.03 512 1.03

This set of particles was used for the final Refine3D of the helically-symmetric ADP-F-actin at EMD-27114 and PDB: 8D13 The run_data.star file corresponds to the final Refine3D that was run using the polished particle stacks in the Polish directory.