REL 2022-07-18 2022-08-12 2022-09-05 0000-0003-1556-4864 Grant J Jensen College of Physical and Mathematical Sciences, Brigham Young University N-181 H ESC Provo UT United States 84602 0000-0003-1556-4864 Grant J Jensen College of Physical and Mathematical Sciences, Brigham Young University N-181 H ESC Provo UT United States 84602 0000-0002-8073-6960 Lauren Ann Metskas Departments of Biological Sciences and Chemistry, College of Science, Purdue University Hockmeyer Hall Room 231, 240 S Martin Jischke Dr West Lafayette IN United States 47907 0000-0003-0042-2257 David F Savage Dept of Molecular & Cell Biology, University of California Berkeley 142 Weill Hall #3200 Berkeley CA United States 94720 Metskas LA Ortega D Oltrogge LM Blikstad C Laughlin T Savage DF Jensen GJ 483.4 10.6019/EMPIAR-11125 EMDB cell EMD-27654 Metskas LA Ortega D Oltrogge LM Blikstad C Laughlin T Savage DF Jensen GJ bioRxiv 2022 10.1101/2022.01.24.477598

This deposition includes: 3 example tomograms with movie frames of purified H. neapolitanus carboxysomes, 3 example tomograms with movie frames of H. neapolitanus cells, associated mdoc files for the tomograms, and the complete set of subtomograms for the Rubisco subtomogram average in EMD-27654.

Metskas LA Ortega D Oltrogge LM Blikstad C Lovejoy DR Laughlin TG Savage DF Jensen GJ Nature communications Nat Commun 1 13 2022 English 10.1038/s41467-022-32584-7 35982043 Reconstructed sample tomograms of purified alpha-carboxysomes, plus frames /data/CBTomograms reconstructed volumes MRC MRC 3 1 32 BIT FLOAT 511 8.832 313 8.832

SIRT-like filtered reconstructed tomograms with bin8 pixel size. These tomograms illustrate different features of alpha-carboxysomes, noted in the accompanying citation. Tomograms have different image heights; see MRC header for more details. Frame stacks (gain-corrected) and mdoc files are included for users wishing to reconstruct in their own programs.

Reconstructed sample tomograms of wild-type H. neapolitanus cells, plus frames /data/HaloTomograms reconstructed volumes MRC MRC 3 1 SIGNED BYTE 1024 8.612 500 8.612

SIRT-reconstructed tomograms with bin4 pixel size. These tomograms illustrate different features of alpha-carboxysomes in vivo, noted in the accompanying citation. Tomograms have different image heights; see MRC header for more details. Frame stacks and mdoc files are included for users wishing to reconstruct in their own programs.

Complete set of subtomograms for Rubisco subtomogram average /data picked particles - multiframe - unprocessed MRC MRC 32930 1 32 BIT FLOAT 160 1.104 160 1.104

subtomograms.tar.gz contains the complete set of Rubisco subtomograms from the purified alpha-carboxysome dataset. The file pattern is subtomograms/particle_*.mrc, and the picked particles file is subtomograms/particles.csv. Subtomograms were cropped in Dynamo from from bin1 WBP tomograms reconstructed in novaCTF. The particles.csv text file contains position and orientation information for each subtomogram, as well as defocus, carboxysome number, cross-correlation coefficient, and other data to allow users to import subtomograms into the software package of their choice. A detailed description of the csv information is in the csvColumnDefinitions.txt file, also in the subtomograms directory.