<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11113" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2022-06-09</depositionDate>
            <releaseDate>2023-01-30</releaseDate>
            <updateDate>2023-02-01</updateDate>
        </keyDates>
        <title>Integration of an event-driven Timepix3 hydrid pixel detector into a cryo-EM workflow</title>
        <correspondingAuthor>
            <authorORCID>0000-0001-6591-4637</authorORCID>
            <firstName>J. Paul</firstName>
            <lastName>van Schayck</lastName>
            <organization type="academic">Division of Nanoscopy, Maastricht MultiModal Molecular Imaging Institute (M4I), FHML, Maastricht University</organization>
            <street>Universiteitssingel 50</street>
            <townOrCity>Maastricht</townOrCity>
            <stateOrProvince>Limburg</stateOrProvince>
            <country>Netherlands</country>
            <postOrZipCode>6229ER</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0001-6056-5888</authorORCID>
            <firstName>Raimond B. G.</firstName>
            <lastName>Ravelli</lastName>
            <organization type="academic">Division of Nanoscopy, Maastricht MultiModal Molecular Imaging Institute (M4I), FHML, Maastricht University</organization>
            <street>Universiteitssingel 50</street>
            <townOrCity>Maastricht</townOrCity>
            <stateOrProvince>Limburg</stateOrProvince>
            <country>Netherlands</country>
            <postOrZipCode>6229 ER</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-6591-4637">van Schayck JP</author>
            <author authorORCID="0000-0003-4482-1866">Zhang Y</author>
            <author authorORCID="0000-0002-7539-1160">Knoops K</author>
            <author authorORCID="0000-0002-2964-5684">Peters PJ</author>
            <author authorORCID="0000-0001-6056-5888">Ravelli RBG</author>
        </authorsList>
        <datasetSize units="TB">2.7</datasetSize>
        <entryDOI>10.6019/EMPIAR-11113</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-15389</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0001-6591-4637" order="1">van Schayck JP</author>
                    <author authorORCID="0000-0003-4482-1866" order="2">Zhang Y</author>
                    <author authorORCID="0000-0002-7539-1160" order="3">Knoops K</author>
                    <author authorORCID="0000-0002-2964-5684" order="4">Peters PJ</author>
                    <author authorORCID="0000-0001-6056-5888" order="5">Ravelli RBG</author>
                    <title>Integration of an event-driven Timepix3 hydrid pixel detector into a cryo-EM workflow</title>
                    <journal>Microscopy &amp; Microanalysis</journal>
                    <journalAbbreviation>MAM</journalAbbreviation>
                    <country>Netherlands</country>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Processed but unaligned Timepix3 data. Processed with CNN-ToT event localisation, chip edge correction, deterministic blur (sigma=0.5) and stored at two times super resolution and gain corrected.</name>
        <directory>/data/mrc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>2980</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>50</frameRangeMax>
        </frameRange>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>1032</imageWidth>
            <pixelWidth>0.6</pixelWidth>
            <imageHeight>1032</imageHeight>
            <pixelHeight>0.6</pixelHeight>
        </dimensions>
        <details>Processed Timepix3 data. Processed with CNN-ToT event localisation, chip edge correction, deterministic blur (sigma=0.5) and stored at two times super resolution. Split into 50 frames. Frames have a fluence of ~1.0 e-/A^2. Frames have been gain corrected.</details>
        <segmentationList/>
        <micrographsFilePattern>data/mrc/frame_*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/particles.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/particles</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Raw, unprocessed Timepix3 data.</name>
        <directory>/data/timepix3</directory>
        <category>micrographs - single frame</category>
        <headerFormat>TPX3</headerFormat>
        <dataFormat>TPX3</dataFormat>
        <numImagesOrTiltSeries>2978</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>512</imageWidth>
            <pixelWidth>1.2</pixelWidth>
            <imageHeight>512</imageHeight>
            <pixelHeight>1.2</pixelHeight>
        </dimensions>
        <details>These are raw, unprocessed Timepix3 data. Recorded 1.5 s at a flux of 29 e/A^2/s with a 1.2 A pixelsize. Processing can be done using tpx3HitParser (https://doi.org/10.5281/zenodo.3641237) and generation of image frames using tpx3EventViewer (https://doi.org/10.5281/zenodo.3693989).</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
