REL
2022-06-15
2022-11-11
2022-11-11
Tilt series of SARS-CoV-2 virions, Wuhan/Alpha/Beta/Delta variants
0000-0002-5716-0270
Thomas
Calcraft
The Francis Crick Institute
1 Midland Road
London
United Kingdom
NW1 1AT
0000-0002-0387-2862
Peter
Rosenthal
The Francis Crick Institute
1 Midland Road
London
United Kingdom
NW1 1AT
Calder LJ
Calcraft T
Hussain S
Harvey R
Rosenthal PB
120.5
10.6019/EMPIAR-11070
EMDB
molecule
EMD-15182
EMD-15183
EMD-15181
EMD-15185
Calder LJ
Calcraft T
Hussain S
Harvey R
Rosenthal PB
Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly
Communications Biology
5
2022
10.1038/s42003-022-04183-1
Unaligned tilt movies of SARS-CoV-2 virions (Wuhan strain)
/data/TiltSeries/Wuhan_Movies
micrographs - multiframe
MRC
MRC
533
4
32 BIT FLOAT
3838
2.22
3710
2.22
Movies have 4 frames each, corresponding to 2.38e-/A^2 per tilt. Tilt series collected +/-60deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is 177deg.
Tilt series of SARS-CoV-2 virions (Alpha variant B.1.1.7)
/data/TiltSeries/Kent_B117
tilt series
MRC
MRC
2
39
UNSIGNED 16 BIT INTEGER
4096
2.58
4096
2.58
2.5e-/A^2 dose per tilt. Tilt series collected +/-57deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.
Tilt series of SARS-CoV-2 virions (Beta variant B.1.351)
/data/TiltSeries/SA_B1351
tilt series
MRC
MRC
2
39
UNSIGNED 16 BIT INTEGER
4096
2.58
4096
2.58
2.5e-/A^2 dose per tilt. Tilt series collected +/-57deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.
Tilt series of SARS-CoV-2 virions (Delta variant B.1.617)
/data/TiltSeries/India_B1617
tilt series
MRC
MRC
2
41
UNSIGNED 16 BIT INTEGER
4096
2.58
4096
2.58
2.0e-/A^2 dose per tilt. Tilt series collected +/-60deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.