<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10972" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2021-11-17</depositionDate>
            <releaseDate>2022-03-28</releaseDate>
            <updateDate>2022-03-28</updateDate>
        </keyDates>
        <title>Representative data from Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of cytoplasmic vesicles and mitochondria during HSV-1 infection</title>
        <correspondingAuthor>
            <authorORCID>0000-0003-4547-4034</authorORCID>
            <firstName>Stephen</firstName>
            <middleName>C</middleName>
            <lastName>Graham</lastName>
            <organization type="academic">Department of Pathology, University of Cambridge</organization>
            <street>Tennis Court Road</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB2 1QP</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0003-4547-4034</authorORCID>
            <firstName>Stephen</firstName>
            <middleName>C</middleName>
            <lastName>Graham</lastName>
            <organization type="academic">Department of Pathology, University of Cambridge</organization>
            <street>Tennis Court Road</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB2 1QP</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator>
            <authorORCID>0000-0001-9918-9998</authorORCID>
            <firstName>Colin</firstName>
            <middleName>M</middleName>
            <lastName>Crump</lastName>
            <organization type="academic">Department of Pathology, University of Cambridge</organization>
            <street>Tennis Court Road</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB2 1QP</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator>
            <authorORCID>0000-0001-8091-9057</authorORCID>
            <firstName>Maria</firstName>
            <lastName>Harkiolaki</lastName>
            <organization type="academic">B24, Diamond Light Source</organization>
            <street>Fermi Avenue</street>
            <townOrCity>Didcot</townOrCity>
            <stateOrProvince>Oxfordshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>OX11 0DE</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-3501-8473">Nahas KLN</author>
            <author>Connor VC</author>
            <author authorORCID="0000-0002-2042-5407">Scherer KM</author>
            <author authorORCID="0000-0002-5194-0962">Kaminski CF</author>
            <author authorORCID="0000-0001-8091-9057">Harkiolaki M</author>
            <author authorORCID="0000-0001-9918-9998">Crump CM</author>
            <author authorORCID="0000-0003-4547-4034">Graham SC</author>
        </authorsList>
        <datasetSize>9.9</datasetSize>
        <entryDOI>10.6019/EMPIAR-10972</entryDOI>
        <experimentType>SXT</experimentType>
    </admin>
    <crossReferences>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0003-3501-8473" order="1">Nahas KLN</author>
                    <author order="2">Connor VC</author>
                    <author authorORCID="0000-0001-8091-9057" order="3">Harkiolaki M</author>
                    <author authorORCID="0000-0001-9918-9998" order="4">Crump CM</author>
                    <author authorORCID="0000-0003-4547-4034" order="5">Graham SC</author>
                    <title>Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of cytoplasmic vesicles and mitochondria during HSV-1 infection</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United Kingdom</country>
                    <year>2021</year>
                    <externalReferences type="doi">10.1101/2021.10.11.463900</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Representative cryoSXT datasets for studying changes to cellular compartments during HSV-1 infection of U2OS cells</name>
        <directory>/data</directory>
        <category>reconstructed volumes</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>14</numImagesOrTiltSeries>
        <framesPerImage>601</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>601</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>946</imageWidth>
            <pixelWidth>100.0</pixelWidth>
            <imageHeight>946</imageHeight>
            <pixelHeight>100.0</pixelHeight>
        </dimensions>
        <details>Representative tomograms from the manuscript "Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of cytoplasmic vesicles and mitochondria during HSV-1 infection" can be found in the hsv_remodelling directory (5 GB).

DOI: 10.1101/2021.10.11.463900

hsv_perinuclear_viral_particle_hff-htert/
KLN_Dec2018_Box14_Grid2_D3F.SXT.FOV1.rec

early_stage_hsv_u2os/SXT/
KLN_July2020_Box1G2_B1F.SXT.FOV3.rec	
KLN_July2020_Box1G2_D3A.SXT.FOV1.rec

hsv_cell_surface_viral_particles_hff-htert/SXT/
KLN_Feb2019_Feb2_Grid3_E3C.SXT.FOV1.rec

hsv_cytoplasmic_viral_particles_hff-htert/SXT/
KLN_Dec2018_Box12_Grid2_E4D.SXT.FOV2.rec

hsv_nuclear_capsids_hff-htert/SXT/
KLN_Feb2019_Feb2G1_D4F.SXT.FOV1.rec

late_stage_hsv_u2os/SXT/
BoxK1p3_Grid4_E5D.SXT.FOV2.rec
KLN_July2020_Box8G1_E2E.SXT.FOV2.rec

uninfected_u2os/SXT/
BoxK1p1_Grid2_A4B.SXT.FOV5.rec
KLN_July2020_Box2_G2_F5A.SXT.FOV1.rec

Tomograms from Supplementary figure 1 of the manuscript "Contour, a semi-automated segmentation and quantitation tool for cryo-soft-X-ray tomography" can be found in the contour_supplementary_figure directory (4.9GB).

DOI: 10.1101/2021.12.03.470962

HFF-hTERT/SXT/
KLN_Oct2020_Box8G2_E1C.SXT.FOV1.rec

HaCaT/SXT/
KLN_Jun2018_Box8G2_E5A.SXT.FOV1.rec

U2OS/SXT/
KLN_July2020_Box9G3_D5C.SXT.FOV1.rec

Vero/SXT/
NV_Box3G3_B3D.SXT.FOV1.rec</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
