Cryo-EM structure of the folate-specific ECF transporter complex in DDM micelles (200 kV)

REL 2022-01-27 2022-02-07 2022-02-07 Cryo-EM structure of the folate-specific ECF transporter complex in DDM micelles (200 kV) 0000-0001-7017-109X Cristina Paulino Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG 0000-0001-7017-109X Cristina Paulino Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG 0000-0002-5804-9689 Dirk Jan Slotboom Faculty of Science and Engineering, Membrane Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG Thangaratnarajah C Rheinberger J Paulino C Slotboom DJ 141.7 10.6019/EMPIAR-10941 EMDB EMD-12483 Thangaratnarajah C Rheinberger J Paulino C Slotboom DJ Insights into the bilayer-mediated toppling mechanism of a folate-specific ECF transporter by cryo-EM Proceedings of the National Academy of Sciences of the United States of America Proc Natl Acad Sci U S A 34 118 2021 English 10.1073/pnas.2105014118 34408021 Unaligned movie frames of ECF-FolT2 in DDM micelles (Dataset 1, Quantifoil grid) /data/raw_190319_ECF-FolT2-bCT2s2_ngc micrographs - multiframe TIFF TIFF 443 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each containing 60 frames per movie collected from a Quantifoil grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 53.3 electrons per Å^2. Movies are not gain corrected and the gain reference is provided.

Unaligned movie frames of ECF-FolT2 in DDM micelles (Dataset 2, UltrAuFoil grid) /data/raw_190318_ECF-FolT2-bCT2s3_ngc micrographs - multiframe TIFF TIFF 265 60 UNSIGNED BYTE 3838 1.012 3710 1.012

Unaligned multi-frame movies with each containing 60 frames per movie collected from a UltrAuFoil grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 53.3 electrons per Å^2. Movies are not gain corrected and the gain reference is provided.