<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10937" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2022-01-19</depositionDate>
            <releaseDate>2022-03-21</releaseDate>
            <updateDate>2022-10-17</updateDate>
        </keyDates>
        <title>CryoEM of PreP prepared via Chameleon</title>
        <correspondingAuthor>
            <authorORCID>0000-0002-8267-8995</authorORCID>
            <firstName>Wei-Jen</firstName>
            <lastName>Tang</lastName>
            <organization type="academic">Ben May Department for Cancer Research, Biological Sciences Division, University of Chicago</organization>
            <street>929 E. 57th Street</street>
            <townOrCity>Chicago</townOrCity>
            <stateOrProvince>Illinois</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>60637</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0002-8267-8995</authorORCID>
            <firstName>Wei-Jen</firstName>
            <lastName>Tang</lastName>
            <organization type="academic">Ben May Department for Cancer Research, Biological Sciences Division, University of Chicago</organization>
            <street>929 E. 57th Street</street>
            <townOrCity>Chicago</townOrCity>
            <stateOrProvince>Illinois</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>60637</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-2143-5893">Liang WG</author>
            <author>Wijaya J</author>
            <author authorORCID="0000-0001-6777-1772">Wei H</author>
            <author authorORCID="0000-0001-8634-2279">Noble AJ</author>
            <author authorORCID="0000-0003-3368-6275">Mancl JM</author>
            <author>Mo S</author>
            <author authorORCID="0000-0001-7232-0968">Lee D</author>
            <author authorORCID="0000-0001-5194-4798">Lin King JV</author>
            <author authorORCID="0000-0001-7216-8193">Pan M</author>
            <author>Liu C</author>
            <author authorORCID="0000-0001-8685-2412">Koehler CM</author>
            <author authorORCID="0000-0001-5832-6060">Zhao M</author>
            <author authorORCID="0000-0003-2394-0831">Potter CS</author>
            <author authorORCID="0000-0002-0624-5020">Carragher B</author>
            <author authorORCID="0000-0002-9073-6809">Li S</author>
            <author authorORCID="0000-0002-8267-8995">Tang WJ</author>
        </authorsList>
        <datasetSize units="TB">2.2</datasetSize>
        <entryDOI>10.6019/EMPIAR-10937</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-22278</emdbEntry>
            <emdbEntry>EMD-22279</emdbEntry>
            <emdbEntry>EMD-22280</emdbEntry>
            <emdbEntry>EMD-22281</emdbEntry>
            <emdbEntry>EMD-22282</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-2143-5893" order="1">Liang WG</author>
                    <author order="2">Wijaya J</author>
                    <author authorORCID="0000-0001-6777-1772" order="3">Wei H</author>
                    <author authorORCID="0000-0001-8634-2279" order="4">Noble AJ</author>
                    <author authorORCID="0000-0003-3368-6275" order="5">Mancl JM</author>
                    <author order="6">Mo S</author>
                    <author authorORCID="0000-0001-7232-0968" order="7">Lee D</author>
                    <author authorORCID="0000-0001-5194-4798" order="8">Lin King JV</author>
                    <author authorORCID="0000-0001-7216-8193" order="9">Pan M</author>
                    <author order="10">Liu C</author>
                    <author authorORCID="0000-0001-8685-2412" order="11">Koehler CM</author>
                    <author authorORCID="0000-0001-5832-6060" order="12">Zhao M</author>
                    <author authorORCID="0000-0003-2394-0831" order="13">Potter CS</author>
                    <author authorORCID="0000-0002-0624-5020" order="14">Carragher B</author>
                    <author authorORCID="0000-0002-9073-6809" order="15">Li S</author>
                    <author authorORCID="0000-0002-8267-8995" order="16">Tang WJ</author>
                    <title>Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>13</volume>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-022-29322-4</externalReferences>
                    <externalReferences type="pubmed">35383169</externalReferences>
                </journalCitation>
            </universalCitation>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0002-2143-5893" order="1">Liang W</author>
                    <author order="2">Wijaya J</author>
                    <author authorORCID="0000-0001-6777-1772" order="3">Wei H</author>
                    <author authorORCID="0000-0001-8634-2279" order="4">Noble AJ</author>
                    <author authorORCID="0000-0003-3368-6275" order="5">Mancl JM</author>
                    <author order="6">Mo S</author>
                    <author order="7">Lee D</author>
                    <author authorORCID="0000-0001-5194-4798" order="8">King JK</author>
                    <author order="9">Pan M</author>
                    <author order="10">Liu C</author>
                    <author authorORCID="0000-0001-8685-2412" order="11">Koehler CM</author>
                    <author authorORCID="0000-0001-5832-6060" order="12">Zhao M</author>
                    <author authorORCID="0000-0003-2394-0831" order="13">Potter CS</author>
                    <author authorORCID="0000-0002-0624-5020" order="14">Carragher B</author>
                    <author order="15">Li S</author>
                    <author authorORCID="0000-0002-8267-8995" order="16">Tang WJ</author>
                    <title>Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition</title>
                    <journal>Research Square</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <year>2020</year>
                    <externalReferences type="doi">10.21203/rs.3.rs-63850/v1</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Apo-PreP micrographs</name>
        <directory>/data/19apr26dRAW</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3247</numImagesOrTiltSeries>
        <framesPerImage>30</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>30</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>7676</imageWidth>
            <pixelWidth>variable</pixelWidth>
            <imageHeight>7420</imageHeight>
            <pixelHeight>variable</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>PreP half particles</name>
        <directory>/data/Micrographs</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>794</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>3710</imageWidth>
            <pixelWidth>variable</pixelWidth>
            <imageHeight>3838</imageHeight>
            <pixelHeight>variable</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Citrate synthase presequence bound PreP</name>
        <directory>/data/19dec09j</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3846</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>variable</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>variable</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Amyloid beta bound PreP</name>
        <directory>/data/19nov20b</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3487</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>variable</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>variable</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
