REL
2021-12-31
2022-02-07
2022-02-07
Cryo-EM multiframe micrographs of human Pol kappa complexed with DNA and monoubiquitylated PCNA
0000-0001-9064-4085
Claudia
Lancey
Department of Molecular & Cell Biology, University of Leicester
University Road
Leicester
Leicestershire
United Kingdom
LE1 7RH
0000-0003-2139-2958
Alfredo
De Biasio
King Abdullah University of Science and Technology
Thuwal 23955
Thuwal
Thuwal
Saudi Arabia
23955
0000-0001-5192-1852
Samir
Hamdan
King Abdullah University of Science and Technology
Thuwal 23955
Thuwal
Thuwal
Saudi Arabia
23955
Lancey C
Tehseen M
Bakshi S
Percival M
Takahashi M
Sobhy MA
Raducanu VS
Blair K
Muskett FW
Ragan TJ
Crehuet R
Hamdan SM
De Biasio A
1.9
10.6019/EMPIAR-10934
EMDB
EMD-12602
Lancey C
Tehseen M
Bakshi S
Percival M
Takahashi M
Sobhy MA
Raducanu VS
Blair K
Muskett FW
Ragan TJ
Crehuet R
Hamdan SM
De Biasio A
Cryo-EM structure of human Pol κ bound to DNA and mono-ubiquitylated PCNA
Nature communications
Nat Commun
1
12
2021
English
10.1038/s41467-021-26251-6
34667155
Images taken grids using a FEI Titan Krios G3 with Gatan K3 camera in super resolution mode (binning 1). Defocus range -2.0 to -0.8 μm. Total dose = 46.6 e-/Å^2.
Multiframe micrographs of human Pol κ complexed with monoubiquitylated PCNA and DNA
/data/CL44-1_20201106_111915
micrographs - multiframe
TIFF
TIFF
3889
45
UNSIGNED BYTE
11520
0.543
8184
0.543
The given pixel size accounts for super resolution binning 1 - the physical pixel size was 1.086 Å/pix. Movies are non-gain normalised - gain reference included.
data/CL44-1_20201106_111915/Images-Disc1/GridSquare_*/Data/FoilHole_*_fractions.tiff.bz2