Anaplastic lymphoma kinase (ALK) extracellular fragment of ligand binding region 648-1025 in complex with AUG-alpha

REL 2022-01-05 2022-01-28 2022-01-28 Anaplastic lymphoma kinase (ALK) extracellular fragment of ligand binding region 648-1025 in complex with AUG-alpha 0000-0003-1751-3964 Andrey V Reshetnyak Department of Structural Biology, St. Jude Children's Research Hospital 262 Danny Thomas Pl Memphis TN United States 38105 0000-0001-6354-2796 Charalampos G Kalodimos Department of Structural Biology, St. Jude Children's Research Hospital 262 Danny Thomas Pl Memphis TN United States 38105 Reshetnyak AV Myasnikov AG Kalodimos CG 10.2 10.6019/EMPIAR-10930 EMDB EMD-24095 Reshetnyak AV Rossi P Myasnikov AG Sowaileh M Mohanty J Nourse A Miller DJ Lax I Schlessinger J Kalodimos CG Mechanism for the activation of the anaplastic lymphoma kinase receptor Nature Nature 600 7887 153 157 2021 English 10.1038/s41586-021-04140-8 34819673

The motion-corrected and CTF-estimated images along with extracted particle stacks and the particle stacks used for final reconstruction

Unaligned multi-frame micrographs of 2:2 ALK:AugAlpha complex /data/Micrographs micrographs - multiframe TIFF TIFF 13618 70 UNSIGNED BYTE 11520 0.413 8184 0.413

Raw movie frames. Movies were recorded at defocus values from −0.8 to −1.8 m at 105,000× magnification, corresponding to a pixel size of 0.826 Å at the specimen level (super-resolution pixel size is 0.413). Seventy frames with 2.8-s exposure (0.04 s per frame and the dose of 1.2 e per frame per A2) were collected with the total dose of around 88.4 e, and the first frame was discarded.

data/Micrographs/Titan_Andrey_S319_G13_V1-1_*.tif data/Particles/particles_*.star data/Particles/ Aligned single-frame micrographs of 2:2 ALK:AugAlpha complex /data/Micrographs_motion_corrected micrographs - single frame MRC MRC 13618 1 32 BIT FLOAT 5760 0.826 4092 0.826

Aligned micrographs. Motion correction was performed on raw super-resolution movie stacks and binned by 2 using MotionCor2 data/Particles/ - Particles were automatically picked using cisTEM. Here we provide two star files, so that someone can extract particles in Relion using motion-corrected images and one of the star files (final structure and all picked particles) with the coordinates. Box size used for reconstruction was 256.

data/Micrographs_motion_corrected/Titan_Andrey_S319_G13_V1-1_*.mrc data/Particles/particles_*.star data/Particles/