REL
2021-12-23
2022-03-04
2022-10-10
Cryo-electron tomographs of mouse hippocampal neurons
0000-0002-4609-2719
Hana
Nedozralova
National Hearth Lung and Blood Institute, National Institute of Health US
Center Drive
Bethesda
Maryland
United States
20892
0000-0002-1594-2821
Naoko
Mizuno
National Heart Lung and Blood Institute, National Institute of Health, US
Center Drive
Bethesda
Maryland
United States
20892
Nedozralova H
Basnet N
Ibiricu I
Bodakuntla S
Biertümpfel C
Mizuno N
88.6
10.6019/EMPIAR-10923
EMDB
cell
EMD-25485
EMD-25486
Nedozralova H
Basnet N
Ibiricu I
Bodakuntla S
Biertümpfel C
Mizuno N
In situ cryo-electron tomography reveals local cellular machineries for axon branch development
The Journal of cell biology
J Cell Biol
4
221
2022
English
10.1083/jcb.202106086
35262630
Tomographic reconstruction of mouse hippocampal axons. Reconstructed bin4 tomograms. Volumes were filtered using the band pass filter. Tomograms were reconstructed in IMOD by weighted back projection.
Nedozralova H
Basnet N
Ibiricu I
Bodakuntla S
Biertümpfel C
Mizuno N
In situ cryo-electron tomography reveals local cellular machineries for axon branch development
bioRxiv
2021
English
10.1101/2021.07.28.454169
Tomographic reconstruction of mouse hippocampal axons. Reconstructed bin4 tomograms. Volumes were filtered using the band pass filter. Tomograms were reconstructed in IMOD by weighted back projection.
Reconstructed 4x binned tomograms of hippocampal neurons.
/data/TS_01-65_hip_bin4
reconstructed volumes
MRC
MRC
63
10
32 BIT FLOAT
928
21.8
928
21.8
Tilt-series were filtered according to the cumulative radiation dose (Grant and Grigorieff, 2015) and aligned on the basis of the patch tracking algorithm using the IMOD ETOMO package (Kremer et al., 1996). Tomograms were reconstructed from aligned stacks using weighted back-projection in IMOD. Tomograms were further 4x binned, resulting in pixel sizes of 21.8 Å. Tomograms were denoised by edge enhancing diffusion (bnad command in Bsoft (Heymann et al., 2008)).