<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10915" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2020-06-17</depositionDate>
            <releaseDate>2022-07-12</releaseDate>
            <updateDate>2022-10-10</updateDate>
        </keyDates>
        <title>CryoEM data of PLA2R at pH 6.2 with both 0 and 30 degree tilts.</title>
        <correspondingAuthor>
            <authorORCID>0000-0003-2289-1281</authorORCID>
            <firstName>Michael</firstName>
            <middleName>Paul</middleName>
            <lastName>Lockhart-Cairns</lastName>
            <organization type="academic">University of Manchester</organization>
            <street>Oxford Road</street>
            <townOrCity>Manchester</townOrCity>
            <stateOrProvince>Greater Manchester</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>M13 9PT</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0003-3497-1959</authorORCID>
            <firstName>Clair</firstName>
            <lastName>Baldock</lastName>
            <organization type="academic">University of Manchester</organization>
            <street>Oxford Road</street>
            <townOrCity>Manchester</townOrCity>
            <stateOrProvince>Greater Manchester</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>M13 9PT</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator>
            <authorORCID>0000-0002-7163-9130</authorORCID>
            <firstName>Rachel</firstName>
            <lastName>Lennon</lastName>
            <organization type="academic">University of Manchester</organization>
            <street>Oxford Road</street>
            <townOrCity>Manchester</townOrCity>
            <stateOrProvince>Greater Manchester</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>M13 9PT</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-2289-1281">Lockhart-Cairns MP</author>
        </authorsList>
        <datasetSize units="TB">2.1</datasetSize>
        <entryDOI>10.6019/EMPIAR-10915</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-14077</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-9530-6392" order="1">Fresquet M</author>
                    <author authorORCID="0000-0003-2289-1281" order="2">Lockhart-Cairns MP</author>
                    <author authorORCID="0000-0002-3494-1939" order="3">Rhoden SJ</author>
                    <author authorORCID="0000-0002-4045-0933" order="4">Jowitt TA</author>
                    <author authorORCID="0000-0002-9793-7339" order="5">Briggs DC</author>
                    <author authorORCID="0000-0003-3497-1959" order="6">Baldock C</author>
                    <author authorORCID="0000-0003-1290-9919" order="7">Brenchley PE</author>
                    <author authorORCID="0000-0001-6400-0227" order="8">Lennon R</author>
                    <title>Structure of PLA2R reveals presentation of the dominant membranous nephropathy epitope and an immunogenic patch</title>
                    <journal>Proceedings of the National Academy of Sciences of the United States of America</journal>
                    <journalAbbreviation>Proc Natl Acad Sci U S A</journalAbbreviation>
                    <country></country>
                    <issue>29</issue>
                    <volume>119</volume>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1073/pnas.2202209119</externalReferences>
                    <externalReferences type="pubmed">35858348</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame images of PLA2R at pH 6.2 with a 30 degree tilt.</name>
        <directory>/data/bi22724-12</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>2523</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.415</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.415</pixelHeight>
        </dimensions>
        <details>Images were collected at 30 degree tilt on a K3 in super res mode with sampling of 0.415 Å/pix. The gain image is in its own directory.
Titan Krios: 300 kV
Cs: 2.7
Frames: 40
Dose: 42.9 e/Å^2
Dose rate: 14.8 e.pix/sec</details>
        <segmentationList/>
        <micrographsFilePattern>data/bi22724-12/FoilHole_26998761_Data_27000655_27000657_20200127_120020_fractions.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multi-frame images of PLA2R at pH 6.2 with 0 degree tilt (not used in publication)</name>
        <directory>/data/bi22724-15</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3703</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.415</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.415</pixelHeight>
        </dimensions>
        <details>Images were collected at 0 degree tilt on a K3 in super res mode with sampling of 0.415 Å/pix. The gain image is in the main directory. This data was not used in the final publication.
Titan Krios: 300 kV
Cs: 2.7
Frames: 40
Dose: 42.9 e/Å^2
Dose rate: 14.8 e.pix/sec</details>
        <segmentationList/>
        <micrographsFilePattern>data/bi22724-15/225-2_0000_Dec13.tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
