FIB-SEM of mouse optic nerve of an inducible conditional Mbp knock-out 26 weeks after induction

REL 2021-12-15 2022-01-12 2022-03-28 FIB-SEM of mouse optic nerve of an inducible conditional Mbp knock-out 26 weeks after induction 0000-0002-2902-7165 Wiebke Möbius Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine Hermann-Rein-Straße 3 Göttingen Lower Saxony Germany D-37075 0000-0002-2902-7165 Wiebke Möbius Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine Hermann-Rein-Straße 3 Göttingen Lower Saxony Germany D-37075 Meschkat M Steyer AM Ruhwedel T Möbius W 21.5 10.6019/EMPIAR-10908 FIB-SEM Meschkat M Steyer AM Weil M Kusch K Jahn O Piepkorn L Agüi-Gonzalez P Ngoc Phan NT Ruhwedel T Sadowski B Rizzoli SO Werner HB Ehrenreich H Nave K Möbius W White matter integrity requires continuous myelin synthesis at the inner tongue bioRxiv 2020 English 10.1101/2020.09.02.279612

The micrographs are obtained by FIB-SEM (Zeiss Crossbeam 540): SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3 nA , z-step 50 nm. Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ

FIB-SEM data stack of mouse optic nerve of a conditional and inducible myelin basic protein (Mbp) knock-out 26 weeks after induction /data/FIB-SEM data set 20171106_rOTO 2117_5x5x50_Align_SIFT/FIB-SEM data set 20171106_rOTO micrographs - single frame TIFF TIFF 747 1 UNSIGNED BYTE 7008 50.0 4416 50.0

Mouse: 26 weeks after tamoxifen-induction, genotype: Mbpfl/fl*PlpCreERT2+, induction at the age of 8 weeks Sample preparation: chemical fixation (2.5 % glutaraldehyde, 4 % formaldehyde, 0.5% NaCl), rOTO embedding, Durcupan FIB-SEM: SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3nA), z-step 50 nm Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ