REL 2021-12-17 2022-01-12 2022-10-17 FIB-SEM of an Mbp-deficient shiverer mouse optic nerve 0000-0002-2902-7165 Wiebke Möbius Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine Hermann-Rein-Straße 3 Göttingen Lower Saxony Germany D-37075 0000-0002-2902-7165 Wiebke Möbius Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine Hermann-Rein-Straße 3 Göttingen Lower Saxony Germany D-37075 Meschkat M Steyer AM Ruhwedel T Möbius W 18.8 10.6019/EMPIAR-10907 FIB-SEM tissue Meschkat M Steyer AM Weil MT Kusch K Jahn O Piepkorn L Agüi-Gonzalez P Phan NTN Ruhwedel T Sadowski B Rizzoli SO Werner HB Ehrenreich H Nave KA Möbius W White matter integrity in mice requires continuous myelin synthesis at the inner tongue Nature communications Nat Commun 1 13 2022 English 10.1038/s41467-022-28720-y 35246535
The micrographs are obtained by FIB-SEM (Zeiss Crossbeam 540): SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3 nA , z-step 50 nm. Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ
 Meschkat M Steyer AM Weil M Kusch K Jahn O Piepkorn L Agüi-Gonzalez P Ngoc Phan NT Ruhwedel T Sadowski B Rizzoli SO Werner HB Ehrenreich H Nave K Möbius W White matter integrity requires continuous myelin synthesis at the inner tongue bioRxiv 2020 10.1101/2020.09.02.279612
The micrographs are obtained by FIB-SEM (Zeiss Crossbeam 540): SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3 nA , z-step 50 nm. Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ
 FIB-SEM data stack of an Mbp-deficient shiverer mouse optic nerve /data/FIB-SEM Data set 20171117_shiverer rOTO micrographs - single frame TIFF TIFF 857 1 UNSIGNED BYTE 4650 50.0 5076 50.0
Optic nerve of a male Mbp-deficient mouse (shiverer), perfusion fixation: 2.5 % glutaraldehyde, 4 % formaldehyde, 0.5% NaCl, rOTO embedding, Durcupan. FIB-SEM: SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 700 pA, z-step 50 nm Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ