REL 2021-12-10 2022-03-07 2022-03-07 0000-0003-2306-7040 Alexandra Pacureanu ESRF - The European Synchrotron 71 Avenue des Martyrs Grenoble Isère France 38000 0000-0003-3551-8283 Stephen Stukins Department of Earth Sciences, the Natural History Museum Cromwell Road London London United Kingdom SW7 5BD Cojocaru R Mannix O Capron M Miller CG Jouneau PH Gallet B Falconet D Pacureanu A Stukins S 25.2 10.6019/EMPIAR-10903 FIB-SEM Cojocaru R Mannix O Capron M Miller CG Jouneau PH Gallet B Falconet D Pacureanu A Stukins S Science advances Sci Adv United States 6 8 2022 English 10.1126/sciadv.abd0892 35138906 HRTEM measurements of modern pine pollen grains /data/HRTEM micrographs - single frame TIFF TIFF 16 1 UNSIGNED 16 BIT INTEGER 2048 variable 2048 variable

Fields of view ranging from 8.7459 microns down to 96.5827 nm. The field of view is indicated at the end of each filename. For more details, please contact the corresponding authors or directly Dr. Pierre-Henri Jouneau at CEA Grenoble.

SEM measurements of modern pine and fossil bisaccate pollen grains /data/SEM micrographs - single frame TIFF TIFF 16 1 UNSIGNED BYTE 3072 variable 2207 variable

See legend in each image for more details concerning the experimental parameters. For further inquiries, please contact Dr. Giles Miller at the Natural History Museum in London.

Confocal microscopy measurements of modern pine and fossil bisaccate pollen grains /data/Confocal microscopy micrographs - multiframe TIFF TIFF 7 1 UNSIGNED 16 BIT INTEGER variable variable variable variable

File format is "nd2" and can be opened using FIJI/ImageJ. For more details, please contact Dr. Stephen Stukins at the Natural History Museum in London.

TEM with chemical fixation measurements of modern pine pollen grains /data/TEM_chemical_fixation micrographs - single frame TIFF TIFF 10 1 UNSIGNED 16 BIT INTEGER variable variable variable variable

See scale bar on each image. For more details, please contact the corresponding authors or directly Dr. Denis Falconet at CEA Grenoble.

FIB-SEM measurements of modern pine pollen grains /data/FIB-SEM micrographs - multiframe TIFF TIFF 3 1 UNSIGNED BYTE variable variable variable variable

File "stack02_": Reduced from 16-bit with pixel size 6 nm to 8-bit with pixel size 12 nm. Bleach correction in FIJI, with histogram normalization option. Width: 3345 pixels. Height: 710 pixels. Number of images: 1184. File "stack03_": Reduced from 16-bit with pixel size 6 nm to 8-bit with pixel size 12 nm. Acquired on the edge of a grain, aligned and inverted. Width: 2557 pixels. Height: 953 pixels. Number of images: 2100. File "stack04_": Reduced from 16-bit with pixel size 18 nm to 8-bit with pixel size 36 nm. Stack aligned, with the contrast reversed, then brightness and contrast slightly adjusted. Width: 2244. Height: 768. Number of images: 171. Also included are SEM measurements of the surface of the block before the FIB-SEM acquisition. The original acquisitions at 16-bit are available upon request from the corresponding authors or directly from Dr. Pierre-Henri Jouneau at CEA Grenoble.

X-ray nano-tomography measurements of modern pine pollen grains - 1st sample - 2 reconstructions /data/X-ray phase contrast holographic nano-tomography (ESRF ID16A)/modernPine_a reconstructed volumes TIFF TIFF 2 1 UNSIGNED BYTE variable 350.0 variable 350.0

Raw data available here: https://doi.esrf.fr/10.15151/ESRF-DC-339743082 Experiment protocol: https://protocols.io/view/id16a-measurements-on-pollen-samples-bpfwmjpe.html File format is "raw". Header information is given in ".info" files. Files can be opened for example using ImageJ/FIJI, using the exatra information described below. File "modernPine_a_" has a voxel size of 35 nm, width: 1322 pixels, height: 1446 pixels, number of images: 295, 8-bit. File "modernPine_a-g2_" has a voxel size of 35 nm, width: 1024 pixels, height: 1024 pixels, number of images: 1024, 8-bit. For more details, please contact the corresponding author, Dr. Alexandra Pacureanu at the ESRF.

X-ray nano-tomography measurements of modern pine pollen grains - 2nd sample - 2 reconstructions /data/X-ray phase contrast holographic nano-tomography (ESRF ID16A)/lycopodiumPine_Pine reconstructed volumes TIFF TIFF 2 1 UNSIGNED BYTE variable 400.0 variable 400.0

Raw data available here: https://doi.esrf.fr/10.15151/ESRF-DC-339743082 Experiment protocol: https://protocols.io/view/id16a-measurements-on-pollen-samples-bpfwmjpe.html File format is "raw". Header information is given in ".info" files. Files can be opened for example using ImageJ/FIJI, using the exatra information described below. File "lycopodiumPine_Pine_" has a voxel size of 40 nm, width: 1452 pixels, height: 1569 pixels, number of images: 711, 8-bit. File "LycopodiumPine_Pine-reconstruction2_" has a voxel size of 40 nm, width: 1900 pixels, height: 1400 pixels, number of images: 1260, 8-bit. For more details, please contact the corresponding author, Dr. Alexandra Pacureanu.

AFM measurements on modern pine and fossil bisaccate pollen grains - raw measurement files /data/AFM/Raw data micrographs - single frame TIFF TIFF 318 1 UNSIGNED BYTE variable variable variable variable

Experiment protocol: https://protocols.io/view/afm-measurements-on-pollen-sample-bn37mgrn.html Raw measurement files contain either tapping mode images or mechanical measurements such as force curves or force maps. File format is ibw (Igor Pro file format). These can be opened in the Igor Pro software. The tapping mode images can also be opened in the Gwyddion software. For more details, please contact the corresponding authors or directly Dr. Marie Capron at the PSCM/ESRF.

AFM measurements on modern pine and fossil bisaccate pollen grains - processed data files /data/AFM/Processed data micrographs - single frame JPEG JPEG 93 1 UNSIGNED 32 BIT INTEGER variable variable variable variable

Experiment protocol: https://protocols.io/view/afm-measurements-on-pollen-sample-bn37mgrn.html The processed AFM data consists of AFM height and phase trace maps and 3D surface reconstructions in jpg format. Also included are force maps in tif and pdf formats. For more details, please contact the corresponding authors or directly Dr. Marie Capron at the PSCM/ESRF.