REL
2021-12-22
2022-01-12
2022-01-12
Motion corrected micrographs - purified Dot/Icm T4SS particles
0000-0003-1750-4793
Melanie
Ohi
University of Michigan, Life Sciences Institute
210 Washtenaw Ave
Ann Arbor
MI
United States
48109
0000-0003-1750-4793
Melanie
Ohi
University of Michigan, Life Sciences Institute
210 Washtenaw Ave
Ann Arbor
MI
United States
48109
Durie CL
Sheedlo MJ
Chung JM
Byrne BG
Su M
Knight T
Swanson M
Lacy DB
Ohi MD
190.4
10.6019/EMPIAR-10893
EMDB
EMD-22070
Durie CL
Sheedlo MJ
Chung JM
Byrne BG
Su M
Knight T
Swanson M
Lacy DB
Ohi MD
Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex
eLife
Elife
2020
English
10.7554/elife.59530
32876045
Images of the Dot/Icm T4SS complexes from wild-type Legionella pneumophila cells were collected on the Thermo Fisher 300 kV Titan Krios with Gatan K2 Summit Direct Electron Detector camera having a nominal pixel size of 1.64 Å. Micrographs were acquired using Leginon software. The total exposure time was 16 s, and frames were recorded every 0.2 s, resulting in a total accumulated dose of 58.1 e- Å−2 using a defocus range of −1 to −3 μm. The video frames were aligned using Motioncor2.
Motion corrected micrographs Legionella pneumophila Dot/Icm T4SS
/data
micrographs - single frame
MRC
MRC
3590
80
SIGNED 32 BIT INTEGER
3838
1.64
3710
1.64
The images of the T4SS complexes from wild-type cells were collected on the Thermo Fisher 300 kV Titan Krios with Gatan K2 Summit Direct Electron Detector (DED) camera having a nominal pixel size of 1.64 Å. Micrographs were acquired using Leginon software. The total exposure time was 16 s, and frames were recorded every 0.2 s, resulting in a total accumulated dose of 58.1 e- Å−2 using a defocus range of −1 to −3 μm. The video frames were aligned using Motioncor2.