REL 2021-11-22 2022-06-17 2022-06-17 0000-0002-9864-3586 James Anthony Letts Department of Cellular and Molecular Biology, University of California, Davis 1 Shields Ave Davis California United States 95616 0000-0002-9864-3586 James Anthony Letts UC Davis, MCB One Shields Ave, MCB Department, Briggs Hall Davis CA United States 95616 Letts JA Zhou L Guo F 9.9 10.6019/EMPIAR-10882 EMDB molecule EMD-32325 Zhou L Maldonado M Padavannil A Guo F Letts JA Science (New York, N.Y.) Science 6595 376 831 839 2022 English 10.1126/science.abn7747 35357889 Unaligned multiframe micrographs of Tetrahymena respiratory chain complexes /data/47483876 micrographs - multiframe TIFF TIFF 10728 75 1 75 UNSIGNED BYTE 11520 0.44 8184 0.44

These images correspond to the unprocessed multi-frame micrographs of three data collections on independent biological and protein isolation replicates of partially purified Tetrahymena mitochondrial respiratory chain complexes. The data were collected on a 200 kV TFS Glacios microscope using a Gatan K3 detector in super-resolution mode. The gain references are also included: SuperRef_lz_tt_bottom_001_Sep18.dm4/mrc corresponds to the data collected on September18-19 (Sep18/19 in image name) and gain.mrc corresponds to the data collected on October02-04 (Oct02/03/04 in image name).

Aligned motion corrected micrographs of Tetrahymena respiratory chain complexes /data/47483876/MotionCorrectedMicrographs micrographs - single frame MRC MRC 10728 1 32 BIT FLOAT 5760 0.88 4092 0.88

These are the motion corrected dose weighted and non-dose weighted micrographs. The unprocessed micrograph stacks were gain corrected, downsamples 2-times and motion corrected using MotionCorr2.