REL 2021-11-14 2022-09-06 2022-09-06 0000-0003-0034-5167 John Francis Hunt Department of Biological Sciences, Columbia University 702A Sherman Fairchild Center, MC2434 New York NY United States 10027 0000-0003-0034-5167 John Francis Hunt Department of Biological Sciences, Columbia University 702A Sherman Fairchild Center, MC2434 New York NY United States 10027 Li YH Zhang RZ Chi W Forouhar F Clarke OB Vorobiev S Singh S Montelione GT Szyperski T Xu Y Hunt JF 902.0 10.6019/EMPIAR-10872 EMDB molecule EMD-32211 EMD-32213 EMD-32212 Li Y Zhang R Wang C Forouhar F Clarke OB Vorobiev S Singh S Montelione GT Szyperski T Xu Y Hunt JF The EMBO journal EMBO J 17 41 2022 English 10.15252/embj.2021108368 35801308

Exposures of 2.5 s were dose-fractionated into 50 frames (50 ms/frame), with an exposure rate of 16 electrons/pixel/sec, resulting in a total exposure of 56.15 electrons/A2. The images were recorded using a K3 camera and imaging filter (Gatan Inc., USA) in counting mode at a nominal magnification of 105,000× (nominal pixel size of 0.844 #A/pixel, which was refined to 0.837 #A/pixel based on calibration of the final ST map against the crystal structure of the SCR/NADPH complex) with a defocus range from -1.34 to -2.17 μm.

Native-SCR-tetramer-frames /data micrographs - multiframe TIFF TIFF 3854 50 UNSIGNED BYTE 5760 0.837 4092 0.837