<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10865" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2021-10-26</depositionDate>
            <releaseDate>2022-02-28</releaseDate>
            <updateDate>2022-02-28</updateDate>
        </keyDates>
        <title>Structural visualization of de novo initiation of RNA polymerase II transcription</title>
        <correspondingAuthor>
            <authorORCID>0000-0002-5552-0463</authorORCID>
            <firstName>Stefan</firstName>
            <lastName>Steimle</lastName>
            <organization type="academic">Department of Biochemistry &amp; Biophysics, Perelman School of Medicine, University of Pennsylvania</organization>
            <street>3205 Walnut St</street>
            <townOrCity>Philadelphia</townOrCity>
            <stateOrProvince>PA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>19104</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0002-2017-5575</authorORCID>
            <firstName>Kenji</firstName>
            <lastName>Murakami</lastName>
            <organization type="academic">Department of Biochemistry &amp; Biophysics, Perelman School of Medicine, University of Pennsylvania</organization>
            <street>415 Curie Blvd</street>
            <townOrCity>Philadelphia</townOrCity>
            <stateOrProvince>PA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>19104</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Yang C</author>
            <author>Fujiwara R</author>
            <author>Kim HJ</author>
            <author>Basnet P</author>
            <author>Zhu Y</author>
            <author>Gorbea Colón JJ</author>
            <author authorORCID="0000-0002-5552-0463">Steimle S</author>
            <author>Garcia BA</author>
            <author>Kaplan CD</author>
            <author authorORCID="0000-0002-2017-5575">Murakami K</author>
        </authorsList>
        <datasetSize units="TB">14.3</datasetSize>
        <entryDOI>10.6019/EMPIAR-10865</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-23904</emdbEntry>
            <emdbEntry>EMD-23905</emdbEntry>
            <emdbEntry>EMD-23906</emdbEntry>
            <emdbEntry>EMD-23907</emdbEntry>
            <emdbEntry>EMD-23908</emdbEntry>
            <emdbEntry>EMD-23789</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Yang C</author>
                    <author order="2">Fujiwara R</author>
                    <author order="3">Kim HJ</author>
                    <author order="4">Basnet P</author>
                    <author order="5">Zhu Y</author>
                    <author order="6">Gorbea Colón JJ</author>
                    <author authorORCID="0000-0002-5552-0463" order="7">Steimle S</author>
                    <author order="8">Garcia BA</author>
                    <author order="9">Kaplan CD</author>
                    <author authorORCID="0000-0002-2017-5575" order="10">Murakami K</author>
                    <title>Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II</title>
                    <journal>Molecular cell</journal>
                    <journalAbbreviation>Mol Cell</journalAbbreviation>
                    <country></country>
                    <issue>3</issue>
                    <volume>82</volume>
                    <firstPage>660</firstPage>
                    <lastPage>676.e9</lastPage>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1016/j.molcel.2021.12.020</externalReferences>
                    <externalReferences type="pubmed">35051353</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>raw micrographs for PIC + ITC maps</name>
        <directory>/data/PIC+ITC</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>15895</numImagesOrTiltSeries>
        <framesPerImage>30</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.53</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.53</pixelHeight>
        </dimensions>
        <details>Raw movies from which maps PIC-1, PIC-2, PIC-3, ITC and TFIIH (weak binding state) were reconstructed. The dataset is split into 4 subsets (image0-3999, image 4000-7999, image8000-11999, image 12000-15895).</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>raw micrographs for EC+EC map</name>
        <directory>/data/EC+EC</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>16614</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.54</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.54</pixelHeight>
        </dimensions>
        <details>Raw movies from which the EC+EC map was reconstructed.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
