REL 2020-08-14 2021-10-29 2021-10-29 0000-0002-5703-2922 Marie-Charlotte Domart Francis Crick Institute, Electron Microscopy STP 1 Midland Road London London United Kingdom NW1 1AT 0000-0002-5703-2922 Marie-Charlotte Domart Francis Crick Institute, Electron Microscopy STP 1 Midland Road London London United Kingdom NW1 1AT Peddie CJ Domart MC Collinson L 1.7 10.6019/EMPIAR-10819 CLEM Krentzel D Laine RF Domart MC Peddie CJ Henriques R Collinson LM Jones ML Raw and processed benchmark FIB SEM data matching Airyscan data deposited on Biostudies English

Raw and processed benchmark FIB SEM data matching Airyscan data deposited on Biostudies

S-BSST707 Raw FIB SEM run 1 part 1 /data/MP014_FIB_Raw_run1_part1 micrographs - single frame TIFF TIFF 1599 1 UNSIGNED 16 BIT INTEGER 12032 50.0 12032 50.0

First part of the raw data (out of 4 in total). Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Raw FIB SEM run 1 part 2 /data/MP014_FIB_Raw_run1_part2 micrographs - single frame TIFF TIFF 1600 1 UNSIGNED 16 BIT INTEGER 12032 50.0 12032 50.0

Second part of the raw data (out of 4 in total). Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Raw FIB SEM run 1 part 3 /data/MP014_FIB_Raw_run1_part3 micrographs - single frame TIFF TIFF 1534 1 UNSIGNED 16 BIT INTEGER 12032 50.0 12032 50.0

Third part of the raw data (out of 4 in total). Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Raw FIB SEM run 2 /data/Raw run 2 micrographs - single frame TIFF TIFF 1261 1 UNSIGNED 16 BIT INTEGER 12032 50.0 12032 50.0

Last part of the raw data (out of 4 in total). Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Aligned full resolution FIB SEM stack in its original orientation /data/MP014_FIB_cropped_XY_5nm micrographs - multiframe TIFF TIFF 1 5938 UNSIGNED BYTE 7000 50.0 965 50.0

Processed and aligned stack of FIB SEM images at 5 nm isotropic resolution in the original orientation of the acquisition

Aligned full resolution FIB SEM stack resliced to match the orientation of the Airyscan data /data/MP014_FIB_cropped_XZ_5nm micrographs - multiframe TIFF TIFF 1 965 UNSIGNED BYTE 5938 50.0 7000 50.0

Processed and aligned stack of FIB SEM images at 5 nm isotropic resolution resliced to match the orientation of the Airyscan data available on Biostudies S-BSST707

Downscaled (10nm) aligned FIB SEM stack resliced to match the Airyscan dataset /data/MP014_FIB_cropped_XZ_10nm micrographs - multiframe TIFF TIFF 1 483 UNSIGNED BYTE 2969 100.0 3500 100.0

Processed and aligned stack of FIB SEM images downscaled to 10 nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies S-BSST707

Downscaled (20nm) aligned FIB SEM stack resliced to match the Airyscan dataset /data/MP014_FIB_cropped_XZ_20nm micrographs - multiframe TIFF TIFF 1 243 UNSIGNED BYTE 1485 200.0 1750 200.0

Processed and aligned stack of FIB SEM images downscaled to 20 nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies S-BSST707