REL
2021-09-02
2021-12-03
2022-01-21
Cryo electron tomograms of mouse DRG axons (dataset 2)
0000-0002-4691-5746
Helen
Elizabeth
Foster
Human Technopole
Viale Rita Levi‑Montalcini, 1
Milan
Italy
20157
0000-0001-7292-5430
Andrew
Carter
MRC Laboratory of Molecular Biology
Cambridge Biomedical Campus, Francis Crick Ave,
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
Foster HE
Ventura Santos C
Carter AP
206.2
10.6019/EMPIAR-10814
EMDB
EMD-12639
EMD-12640
EMD-13598
EMD-13599
Foster HE
Ventura Santos C
Carter AP
A cryo-ET survey of microtubules and intracellular compartments in mammalian axons
Journal of Cell Biology
J. Cell Biol.
United Kingdom
2
221
2021
English
10.1083/jcb.202103154
34878519
Raw image frames of mouse dorsal root ganglion axons (dataset 2)
/data/Raw_frames
micrographs - multiframe
TIFF
TIFF
39
10
UNSIGNED 16 BIT INTEGER
3710
2.75
3838
2.75
Raw image frames were not gain or defect corrected. 10 frames were acquired per tilt. The gain reference is provided as gainref.dm4. The defects file is provided as defects.txt. The dose per tilt was 1.85 e/Å^2. SerialEM Mdoc files are provided in a separate folder (Mdoc_files) and can be used to generate stacks from the raw image frames. The pixel spacing of the raw image frames is 2.75 Å/pixel. The pixel spacing in the header of the raw image frames and in the Mdoc files (for stacks binned by 4) is inaccurate.
data/Raw_frames/TS_*.tif
Corrected, aligned, dose-filtered and order-sorted tilt series for mouse dorsal root ganglion axons (dataset 2)
/data/Dose-filt_stacks
tilt series
MRC
MRC
39
61
SIGNED 16 BIT INTEGER
3710
2.75
3838
2.75
Corrected, aligned, dose-filtered and order-sorted tilt series. The tilt series were generated from the raw frame images by correction for gain, defects and motion. In addition, tilt images were dose-filtered. Gain-correction and defects files are provided in the Raw_frames folder. Motion-correction was performed with alignframes (IMOD) operated in subTOM.
Tomograms of mouse dorsal root ganglion axons (dataset 2) binned by 4 and deconvolved
/data/bin4_deconv_tomograms
reconstructed volumes
MRC
MRC
39
1
32 BIT FLOAT
959
11.0
927
11.0
Reconstructed bin4 tomograms. Volumes were filtered using the Wiener-like deconvolution filter implemented in WARP (Tegunov 2018, https://github.com/dtegunov/tom_deconv). Tomograms were reconstructed in IMOD by weighted back projection.