REL 2021-08-11 2022-01-18 2022-01-18 0000-0002-0017-2414 Andriko von Kügelgen Sir William Dunn School of Pathology, University of Oxford South Parks Road Oxford Oxfordshire United Kingdom OX1 3RE 0000-0002-0168-0277 Tanmay AM Bharat Sir William Dunn School of Pathology, University of Oxford South Parks Road Oxford Oxfordshire United Kingdom OX1 3RE von Kugelgen A Bharat TAM 304.4 10.6019/EMPIAR-10790 EMDB EMD-13355 Herdman M von Kügelgen A Kureisaite-Ciziene D Duman R El Omari K Garman EF Kjaer A Kolokouris D Löwe J Wagner A Stansfeld PJ Bharat TAM Structure Structure 2021 English 10.1016/j.str.2021.10.012 34800371

The micrographs are the motion-corrected and dose-weighted averages of the raw 20 movie frames (accumulating 44.8 electrons per squared Angstrom in a 8 second exposure). The raw movies were collected using EPU and saved as non-gain corrected MRC files, which have been compressed with 'relion_convert_to_tif' before deposition (see https://relion.readthedocs.io/en/latest/Reference/MovieCompression.html). The original gain reference for the tilted and non-tilted data set have been deposited as well.

Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours (converted from .mrc into .tif with relion_convert_to_tiff) /data/EMPAIR/movies_non_tilted micrographs - multiframe TIFF TIFF 903 20 UNSIGNED 16 BIT INTEGER 3838 1.08 3710 1.08

Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours (converted from .mrc into .tif with relion_convert_to_tiff). The movies contain 20 frames accumulating 44.8 electrons per squared Angstrom in a 8 second exposure. The raw movies were collected using EPU and saved as non-gain corrected MRC files, which have been compressed with 'relion_convert_to_tif' before deposition (see https://relion.readthedocs.io/en/latest/Reference/MovieCompression.html).

data/EMPAIR/movies_non_tilted/FoilHole_*_Data_*_*_*_*-*.tif Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt (converted from .mrc into .tif with relion_convert_to_tiff) /data/EMPAIR/movies_tilted micrographs - multiframe TIFF TIFF 1115 20 UNSIGNED 16 BIT INTEGER 3838 1.08 3710 1.08

Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt (converted from .mrc into .tif with relion_convert_to_tiff). The movies contain 20 frames accumulating 44.8 electrons per squared Angstrom in a 8 second exposure. The raw movies were collected using EPU and saved as non-gain corrected MRC files, which have been compressed with 'relion_convert_to_tif' before deposition (see https://relion.readthedocs.io/en/latest/Reference/MovieCompression.html).

data/EMPAIR/movies_tilted/FoilHole_*_Data_*_*_*_*-*.tif Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours /data/EMPAIR/micrographs_non_tilted micrographs - single frame MRC MRC 903 1 32 BIT FLOAT 3838 1.08 3710 1.08

Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours.

data/EMPAIR/micrographs_non_tilted/FoilHole_*_Data_*_*_*_*-*.mrc Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt /data/EMPAIR/micrographs_tilted micrographs - single frame MRC MRC 1115 1 32 BIT FLOAT 3838 1.08 3710 1.08

Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt.

data/EMPAIR/micrographs_tilted/FoilHole_*_Data_*_*_*_*-*.mrc Gain reference image of the non-tilted and tilted dataset in MRC format /data/EMPAIR/gain_ref micrographs - single frame MRC MRC 2 1 32 BIT FLOAT 3838 1.08 3710 1.08

Gain reference image of the non-tilted and tilted dataset in MRC format