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        <keyDates>
            <depositionDate>2021-07-21</depositionDate>
            <releaseDate>2022-01-07</releaseDate>
            <updateDate>2022-01-07</updateDate>
        </keyDates>
        <title>Cryo-electron tomography of rod outer segments in ABCA4 knockout mice acquired with Volta phase plate in focus</title>
        <correspondingAuthor>
            <authorORCID>0000-0003-2331-4638</authorORCID>
            <firstName>Matthias</firstName>
            <lastName>Poege</lastName>
            <organization type="academic">Max-Planck-Institute of Biochemistry</organization>
            <street>Am Klopferspitz 18</street>
            <townOrCity>Martinsried</townOrCity>
            <stateOrProvince>Bavaria</stateOrProvince>
            <country>Germany</country>
            <postOrZipCode>82152</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0001-8154-8809</authorORCID>
            <firstName>Baumeister</firstName>
            <lastName>Wolfgang</lastName>
            <organization type="academic">Max-Planck-Institute of Biochemistry</organization>
            <street>Am Klopferspitz 18</street>
            <townOrCity>Martinsried</townOrCity>
            <stateOrProvince>Bavaria</stateOrProvince>
            <country>Germany</country>
            <postOrZipCode>82152</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-2331-4638">Poege M</author>
            <author authorORCID="0000-0001-6968-041X">Mahamid J</author>
            <author authorORCID="0000-0001-9884-2123">Imanishi SS</author>
            <author authorORCID="0000-0002-6402-8315">Plitzko JM</author>
            <author authorORCID="0000-0002-0788-545X">Palczewski K</author>
            <author authorORCID="0000-0001-8154-8809">Baumeister W</author>
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        <entryDOI>10.6019/EMPIAR-10771</entryDOI>
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            <emdbEntry>EMD-13324</emdbEntry>
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                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-2331-4638" order="1">Pöge M</author>
                    <author authorORCID="0000-0001-6968-041X" order="2">Mahamid J</author>
                    <author authorORCID="0000-0001-9884-2123" order="3">Imanishi SS</author>
                    <author authorORCID="0000-0002-6402-8315" order="4">Plitzko JM</author>
                    <author authorORCID="0000-0002-0788-545X" order="5">Palczewski K</author>
                    <author authorORCID="0000-0001-8154-8809" order="6">Baumeister W</author>
                    <title>Determinants shaping the nanoscale architecture of the mouse rod outer segment</title>
                    <journal>eLife</journal>
                    <journalAbbreviation>Elife</journalAbbreviation>
                    <country></country>
                    <volume>10</volume>
                    <year>2021</year>
                    <language>English</language>
                    <externalReferences type="doi">10.7554/elife.72817</externalReferences>
                    <externalReferences type="pubmed">34931611</externalReferences>
                </journalCitation>
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        <name>Tomograms of rod outer segments in ABCA4 knockout mice acquired with Volta phase plate</name>
        <directory>/data/VPP_ko_180410</directory>
        <category>reconstructed volumes</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>2</numImagesOrTiltSeries>
        <framesPerImage>400</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>928</imageWidth>
            <pixelWidth>10.48</pixelWidth>
            <imageHeight>928</imageHeight>
            <pixelHeight>10.48</pixelHeight>
        </dimensions>
        <details>4x binned and dose filtered tomograms with dimensions 928x928x464. Tilt series were acquired conventionally with defocus. Projection images were recorded between -60° and 60° with an increment of 2°. The 400 frames are distributed over all projections. The total exposure per tilt-series was ~ 100e-/A^2.</details>
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