REL 2021-05-17 2021-06-25 2021-06-25 0000-0003-3327-1926 Ci Ji Lim UW-Madison Madison Wisconsin United States 53706 0000-0001-7338-3389 Thomas Cech CU Boulder Boulder Colorado United States 80303 Lim C Barbour AT Zaug AJ Goodrich KJ McKay AE Wuttke DS Cech TR 14.3 10.6019/EMPIAR-10718 EMDB EMD-21567 Lim C Barbour AT Zaug AJ Goodrich KJ McKay AE Wuttke DS Cech TR Science (New York, N.Y.) Science 6495 368 1081 1085 2020 English 10.1126/science.aaz9649 32499435

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Unaligned movies of CST with 3xTEL DNA oligomer at 0 degrees stage tilt /data/CST_0tilt_movies micrographs - multiframe TIFF TIFF 20808 60 UNSIGNED BYTE 11520 0.539 8184 variable

This dataset was collected on a KRIOS with 3x3 beam tilt strategy using SerialEM. The optical groups are named just before the date. For example, W3_3_999_009_Aug02_00.49.32.tif is group 9. In addition, this dataset was collected at 0 degrees stage tilt with a pixel size of 0.539 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 60.0 e-/Å2 (60 frames) using a Gatan K3 direct electron detector in super-resolution CDS mode. Gain reference to be applied.

Unaligned movies of CST with 3xTEL DNA oligomer at 30 degrees stage tilt /data/CST_30tilt_movies micrographs - multiframe TIFF TIFF 7227 60 UNSIGNED BYTE 11520 0.539 8184 variable

This dataset was collected on a KRIOS with 3x3 beam tilt strategy using SerialEM. The optical groups are named just before the date. For example, W3_3_30tilt_3315_004_Aug08_10.16.04.tif is group 4. In addition, this dataset was collected at 30 degrees stage tilt with a pixel size of 0.539 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 60.0 e-/Å2 (60 frames) using a Gatan K3 direct electron detector in super-resolution CDS mode. Gain reference to be applied.

Gain reference for both datasets - gainref_20190729.mrc /data micrographs - single frame MRC MRC 1 1 UNSIGNED BYTE 11520 0.539 8184 variable

Gain reference for the two datasets.