Transmission electron micrographs of mouse RNF213 incubated with ATPγS

REL 2020-06-17 2022-06-14 2022-06-14 Transmission electron micrographs of mouse RNF213 incubated with ATPγS 0000-0003-0293-1063 Juraj Ahel IMP Vienna Campus-Vienna-Biocenter 1 Vienna Vienna Austria 1030 0000-0003-1582-6924 Tim Clausen IMP Vienna Vienna Austria 1030 Ahel J Weis F Clausen T 2.3 10.6019/EMPIAR-10712 EMDB molecule EMD-12932 Ahel J Clausen T Virdee S Fletcher AJ Grabarczyk DB Deszcz L Lehner A Roitinger E E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP BiorXiv unaligned multi-frame micrographs from a dataset collected without grid tilting /data/set1_noTilt/raw_micrographs micrographs - multiframe TIFF TIFF 5950 40 1 40 UNSIGNED 16 BIT INTEGER 3838 1.04 3710 1.04

set1_noTilt/raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected == set1_noTilt/set1_GainReference.mrc == gain reference for the dataset set1_noTilt/set1_micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs set1_noTilt/micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs == set1_noTilt/coordinates_initial_cryolo/ == particle coordinates picked by cryolo set1_noTilt/coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction set1_noTilt/particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1 == ./refined_particles_combined.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment == ./cryolo_YOLO_RNF213.h5 == cryolo network model used for initial particles picking ./cryolo_YOLO_RNF213_config.json == config file for the cryolo network model used for initial particles picking ./MTF_K2summit_300kV.star == MTF file used for MTF correction for this dataset

data/set1_noTilt/micrographs_ctf_motioncorr/*mrc data/refined_particles_combined.star data/set1_noTilt/particles unaligned multi-frame micrographs from a dataset collected with a 30 deg grid tilt /data/set2_withTilt/raw_micrographs micrographs - multiframe TIFF TIFF 3055 40 1 40 UNSIGNED 16 BIT INTEGER 5760 1.052 4092 1.052

set2_withTilt/raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected == set2_withTilt/set2_GainReference.mrc == gain reference for the dataset set2_withTilt/set2_micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs set2_withTilt/micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs == set2_withTilt/coordinates_initial_3Dpick/ == particle coordinates picked by the relion 3D template picker set2_withTilt/coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction set2_withTilt/particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1 == ./refined_particles_combined.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment == ./mtf_k3_standard_300kV_FL2.star == MTF file used for MTF correction for this dataset

data/set2_withTilt/micrographs_ctf_motioncorr/*mrc data/refined_particles_combined.star data/set2_withTilt/particles