<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10708" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2021-05-07</depositionDate>
            <releaseDate>2021-10-19</releaseDate>
            <updateDate>2022-02-28</updateDate>
        </keyDates>
        <title>Apoferritin, TMV and T20S proteasome in nanofluidic channels</title>
        <correspondingAuthor>
            <authorORCID>0000-0003-3721-5104</authorORCID>
            <firstName>Stefan</firstName>
            <middleName>T.</middleName>
            <lastName>Huber</lastName>
            <organization type="academic">Department of Bionanoscience / TU Delft</organization>
            <street>Van der Maasweg 9</street>
            <townOrCity>Delft</townOrCity>
            <stateOrProvince>Zuid-Holland</stateOrProvince>
            <country>Netherlands</country>
            <postOrZipCode>2629HZ</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0002-7761-2027</authorORCID>
            <firstName>Arjen</firstName>
            <middleName>J.</middleName>
            <lastName>Jakobi</lastName>
            <organization type="academic">Department of Bionanoscience / TU Delft</organization>
            <street>Van der Maasweg 9</street>
            <townOrCity>Delft</townOrCity>
            <stateOrProvince>Zuid-Holland</stateOrProvince>
            <country>Netherlands</country>
            <postOrZipCode>2629HZ</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-3721-5104">Huber ST</author>
            <author authorORCID="0000-0002-6184-3234">Sarajlic E</author>
            <author authorORCID="0000-0001-7355-941X">Huijink R</author>
            <author authorORCID="0000-0002-8137-3532">Weis F</author>
            <author authorORCID="0000-0002-3413-5128">Evers WH</author>
            <author authorORCID="0000-0002-7761-2027">Jakobi AJ</author>
        </authorsList>
        <datasetSize>308.3</datasetSize>
        <entryDOI>10.6019/EMPIAR-10708</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-12901</emdbEntry>
            <emdbEntry>EMD-12903</emdbEntry>
            <emdbEntry>EMD-12915</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-3721-5104" order="1">Huber ST</author>
                    <author authorORCID="0000-0002-6184-3234" order="2">Sarajlic E</author>
                    <author authorORCID="0000-0001-7355-941X" order="3">Huijink R</author>
                    <author authorORCID="0000-0002-8137-3532" order="4">Weis F</author>
                    <author authorORCID="0000-0002-3413-5128" order="5">Evers WH</author>
                    <author authorORCID="0000-0002-7761-2027" order="6">Jakobi AJ</author>
                    <title>Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes</title>
                    <journal>eLife</journal>
                    <journalAbbreviation>Elife</journalAbbreviation>
                    <country></country>
                    <volume>11</volume>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.7554/elife.72629</externalReferences>
                    <externalReferences type="pubmed">35060902</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame movies of pyrococcus furiosus apoferritin in silicon nitride nanochannels</name>
        <directory>/data/ApoFtn</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>948</numImagesOrTiltSeries>
        <framesPerImage>90</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>0.8127</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>0.8127</pixelHeight>
        </dimensions>
        <details>Movies are not gain-corrected
gain reference is in data/ApoFtn/CountRef_map_0044_001.mrc
needs to be flipped in y direction
defect file is in data/ApoFtn/defects_map_0044_001.txt
3.4 mg/mL concentration of sample
63 e-/A**2 total exposure</details>
        <segmentationList/>
        <micrographsFilePattern>data/ApoFtn/chip*/*tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Multi-frame movies of T20S proteasome in silicon nitride nanochannels</name>
        <directory>/data/T20S</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>121</numImagesOrTiltSeries>
        <framesPerImage>73</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.288</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.288</pixelHeight>
        </dimensions>
        <details>Gain-corrected compressed tif movies
1.4 mg/mL concentration of sample
59.7 e-/A**2 total exposure</details>
        <segmentationList/>
        <micrographsFilePattern>data/T20S/*tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Multi-frame movies of TMV in silicon nitride nanochannels</name>
        <directory>/data/TMV</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>82</numImagesOrTiltSeries>
        <framesPerImage>73</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.288</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.288</pixelHeight>
        </dimensions>
        <details>Gain-corrected compressed tif movies
1.1 mg/mL concentration of sample
59.7 e-/A**2 total exposure</details>
        <segmentationList/>
        <micrographsFilePattern>data/TMV/*tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
