<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10689" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2021-04-07</depositionDate>
            <releaseDate>2023-01-16</releaseDate>
            <updateDate>2023-01-16</updateDate>
        </keyDates>
        <title>Cryo-electron tomography of FIB-milled Caulobacter crescentus expressing PopZ with IDR-156 and pentavalent OD</title>
        <correspondingAuthor>
            <authorORCID>0000-0001-8228-2133</authorORCID>
            <firstName>Vinson</firstName>
            <lastName>Lam</lastName>
            <organization type="academic">UC San Diego</organization>
            <street>9500 Gilman Dr.</street>
            <townOrCity>La Jolla</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>92093</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0003-4677-9809</authorORCID>
            <firstName>Elizabeth</firstName>
            <lastName>Villa</lastName>
            <organization type="academic">UC San Diego</organization>
            <street>9500 Gilman Dr.</street>
            <townOrCity>La Jolla</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>92093</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-5480-4173">Lasker K</author>
            <author authorORCID="0000-0001-8228-2133">Lam V</author>
            <author authorORCID="0000-0003-4677-9809">Villa E</author>
        </authorsList>
        <datasetSize>5.9</datasetSize>
        <entryDOI>10.6019/EMPIAR-10689</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>cell</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-23624</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-5480-4173" order="1">Lasker K</author>
                    <author authorORCID="0000-0002-9705-9871" order="2">Boeynaems S</author>
                    <author authorORCID="0000-0001-8228-2133" order="3">Lam V</author>
                    <author order="4">Scholl D</author>
                    <author authorORCID="0000-0002-4247-8059" order="5">Stainton E</author>
                    <author authorORCID="0000-0002-4760-5037" order="6">Briner A</author>
                    <author authorORCID="0000-0003-2626-4189" order="7">Jacquemyn M</author>
                    <author authorORCID="0000-0001-7092-1153" order="8">Daelemans D</author>
                    <author order="9">Deniz A</author>
                    <author authorORCID="0000-0003-4677-9809" order="10">Villa E</author>
                    <author authorORCID="0000-0002-4155-5729" order="11">Holehouse AS</author>
                    <author authorORCID="0000-0001-8603-1526" order="12">Gitler AD</author>
                    <author authorORCID="0000-0002-0445-067X" order="13">Shapiro L</author>
                    <title>The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Comm</journalAbbreviation>
                    <country>United States</country>
                    <issue>1</issue>
                    <volume>13</volume>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-022-33221-z</externalReferences>
                    <externalReferences type="pubmed">36163138</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned tilt series of FIB-milled Caulobacter crescentus expressing PopZ with IDR-156 and pentavalent OD</name>
        <directory>/data/stack</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>1</numImagesOrTiltSeries>
        <framesPerImage>51</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>51</frameRangeMax>
        </frameRange>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>3710</imageWidth>
            <pixelWidth>4.265</pixelWidth>
            <imageHeight>3838</imageHeight>
            <pixelHeight>4.265</pixelHeight>
        </dimensions>
        <details>Tilt-series is motion-corrected using Warp. This tilt-series was generated with the first three frames of each tilt micrograph removed.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Multi-frame micrographs of each tilt for tilt-series of FIB-milled Caulobacter crescentus expressing PopZ with IDR-156 and pentavalent OD</name>
        <directory>/data/frames</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>51</numImagesOrTiltSeries>
        <framesPerImage>15</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>15</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3710</imageWidth>
            <pixelWidth>4.265</pixelWidth>
            <imageHeight>3838</imageHeight>
            <pixelHeight>4.265</pixelHeight>
        </dimensions>
        <details>Dark-subtracted frames from serialEM. mdoc metadata file and counting mode gain-reference is included in the same directory.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
