REL
2021-02-02
2021-07-30
2021-07-30
Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity
0000-0003-4247-4303
Ricardo
Righetto
Helmholtz Zentrum München
Munich
Germany
85764
0000-0002-0941-4387
Benjamin
D.
Engel
Helmholtz Pioneer Campus
Munich
Germany
85764
Gupta TK
Klumpe S
Gries K
Strauss M
Rudack T
Schuller JM
Schroda M
Engel BD
2.0
10.6019/EMPIAR-10680
EMDB
EMD-12712
Gupta TK
Klumpe S
Gries K
Heinz S
Wietrzynski W
Ohnishi N
Niemeyer J
Spaniol B
Schaffer M
Rast A
Ostermeier M
Strauss M
Plitzko JM
Baumeister W
Rudack T
Sakamoto W
Nickelsen J
Schuller JM
Schroda M
Engel BD
Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity
Cell
Cell
14
184
3643
3659.e23
2021
English
10.1016/j.cell.2021.05.011
34166613
Raw movie data from Titan Krios operated at 300 kV with a K2 direct electron detector operated in counting mode. Pixel size is 1.35 Å.
Unaligned frames of VIPP1 from K2 operated in counting mode
/data/frames
micrographs - multiframe
TIFF
TIFF
8120
80
UNSIGNED BYTE
3838
1.35
3710
1.35
Particle coordinates correspond to initial picking using crYOLO. Micrographs where particles were not detected do not have an associated .box file.
The gain reference is provided in MRC format inside the data/gain/ directory.
Data acquisition details:
Images were acquired on a Titan Krios microscope (FEI, Thermo Fisher Scientific) at 300 kV, using a post-column energy filter (Quantum, Gatan) and a K2 Summit direct electron detector (Gatan) operated in counting mode. For each image, 80 frames were acquired over 8 sec, with a total dose of ~45 e−/Å2 and a calibrated pixel size of 1.35 Å. A total of 8120 images were recorded, using a defocus range of −0.5 to −3.5 μm.
data/frames/*.tif
data/frames/*.box
data/frames