REL
2021-03-31
2021-06-18
2021-06-18
Subtomograms of nucleosomes extracted from cryo-tomograms of Drosophila melanogaster embryos
0000-0003-2298-8964
Mikhail
Eltsov
Institute of Genetics and Molecular and Cellular Biology: Strasbourg, FR
1 rue Laurent Fries
Illkirch
France
67404
0000-0003-2298-8964
Mikhail
Eltsov
Institute of Genetics and Molecular and Cellular Biology: Strasbourg, FR
1 rue Laurent Fries
Illkirch
France
67404
0000-0001-5112-2743
Slavica
Jonic
Bioinformatique et BioPhysique (BIBIP), Institut de minéralogie, de physique des matériaux et de cosmochimie, Sorbonne Université/CNRS/MNHN/IRD
4 place Jussieu
Paris
France
75005
0000-0002-0225-2776
Amélie
Leforestier
Structure et dynamique d’objets biologiques autoassemblés - SOBIO, Laboratoire de Physique des Solides - UMR 8502,
1 rue Nicolas Appert
Orsay
France
91405
Harastani M
Eltsov M
Leforestier A
Jonic S
666.3
10.6019/EMPIAR-10679
EMDB
EMD-12699
Harastani M
Eltsov M
Leforestier A
Jonic S
HEMNMA-3D: Cryo Electron Tomography Method Based on Normal Mode Analysis to Study Continuous Conformational Variability of Macromolecular Complexes
Frontiers in molecular biosciences
Front Mol Biosci
8
2021
English
10.3389/fmolb.2021.663121
34095222
Unaligned subtomograms of nucleosomes extracted from in situ cryo-tomograms of Drosophila melanogaster embryonic brain
/data
subtomograms
MRC
EM
1
6
32 BIT FLOAT
variable
4.4
variable
4.4
Tilt series were recorded using the dose-symmetric recording scheme of Serial EM software at a nominal magnification of 64000 and a target defocus of -3.5 ˚ µm. The total dose was set to 91.5 e−/ A˚
A marker-less tilt series alignment was done in IMOD. Nucleosomes were manually picked in IMOD, and 64x64x64 voxel subtomograms (voxel size of 4.4 A) were extracted from the volumes.