<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10659" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2021-03-03</depositionDate>
            <releaseDate>2021-03-26</releaseDate>
            <updateDate>2021-05-26</updateDate>
        </keyDates>
        <title>GluK2/K5 with 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX)</title>
        <correspondingAuthor>
            <authorORCID>0000-0002-6127-0093</authorORCID>
            <firstName>Joel</firstName>
            <lastName>Meyerson</lastName>
            <organization type="academic">Weill Cornell Medical College</organization>
            <townOrCity>New York</townOrCity>
            <country>United States</country>
            <postOrZipCode>10065</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0002-6127-0093</authorORCID>
            <firstName>Joel</firstName>
            <lastName>Meyerson</lastName>
            <organization type="academic">Weill Cornell Medical College</organization>
            <townOrCity>New York</townOrCity>
            <stateOrProvince>NY</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>10065</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-4217-1273">Khanra NK</author>
            <author authorORCID="0000-0002-6127-0093">Meyerson JRM</author>
        </authorsList>
        <datasetSize units="TB">5.9</datasetSize>
        <entryDOI>10.6019/EMPIAR-10659</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-23014</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-4217-1273" order="1">Khanra N</author>
                    <author authorORCID="0000-0001-8340-0330" order="2">Brown PM</author>
                    <author authorORCID="0000-0001-9681-3548" order="3">Perozzo AM</author>
                    <author authorORCID="0000-0001-9491-8768" order="4">Bowie D</author>
                    <author authorORCID="0000-0002-6127-0093" order="5">Meyerson J</author>
                    <title>Architecture and structural dynamics of the heteromeric GluK2/K5 kainate receptor</title>
                    <journal>eLife</journal>
                    <journalAbbreviation>Elife</journalAbbreviation>
                    <country>United States</country>
                    <volume>10</volume>
                    <year>2021</year>
                    <language>English</language>
                    <externalReferences type="doi">10.7554/elife.66097</externalReferences>
                    <externalReferences type="pubmed">33724189</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>GluK2/K5 with 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX)</name>
        <directory>/data/K2em2_K5em_1mM_CNQX_NYU_Arctica_2020_02_20</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3996</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.548</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.548</pixelHeight>
        </dimensions>
        <details>Dose per frame (e/A2) = 1.3055

The coordinate files for the deposited GluK2-K5-CNQX-full-length, GluK2-K5-CNQX-ATD, and GluK2-K5-CNQX-LBD-TMD maps are provided in STAR and CS file format.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>GluK2/K5 with 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX)</name>
        <directory>/data/K2em2_K5em_1mM_CNQX_NYU_Arctica_2020_02_20_2</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>2770</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.548</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.548</pixelHeight>
        </dimensions>
        <details>Dose per frame (e/A2) = 1.3055</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>GluK2/K5 with 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX)</name>
        <directory>/data/K2em2_K5em_1mM_CNQX_NYU_Arctica_2020_03_04</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>2310</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.548</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.548</pixelHeight>
        </dimensions>
        <details>Dose per frame = 1.33925</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>GluK2/K5 with 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX)</name>
        <directory>/data/GluK2-K5-CNQX-ATD-subtracted-particles</directory>
        <category>picked particles - single frame - processed</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>1021916</numImagesOrTiltSeries>
        <framesPerImage>510958</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>320</imageWidth>
            <pixelWidth>1.096</pixelWidth>
            <imageHeight>320</imageHeight>
            <pixelHeight>1.096</pixelHeight>
        </dimensions>
        <details>The subtracted particle stacks were provided as two .mrcs (half1 and half2) files. The coordinate files for the GluK2/K5-CNQX-ATD map were provided with this deposition. The coordinate files contain the final map particles, which is the subset of total particle stacks deposited.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>GluK2/K5 with 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX)</name>
        <directory>/data/GluK2-K5-CNQX-LBD-TMD-subtracted-particles</directory>
        <category>picked particles - single frame - processed</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>490046</numImagesOrTiltSeries>
        <framesPerImage>245023</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>320</imageWidth>
            <pixelWidth>1.096</pixelWidth>
            <imageHeight>320</imageHeight>
            <pixelHeight>1.096</pixelHeight>
        </dimensions>
        <details>The subtracted particle stacks were provided as two .mrcs (half1 and half2) files. The coordinate files for the GluK2/K5-CNQX-LBD-TMD map were provided with this deposition. The coordinate files contain the final map particles, which is the subset of total particle stacks deposited.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
