<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10654" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2021-03-01</depositionDate>
            <releaseDate>2021-03-24</releaseDate>
            <updateDate>2021-03-30</updateDate>
        </keyDates>
        <title>Alpha-E-catenin ABD-F-actin complex</title>
        <correspondingAuthor>
            <authorORCID>0000-0001-7250-4484</authorORCID>
            <firstName>Gregory</firstName>
            <middleName>Matthew</middleName>
            <lastName>Alushin</lastName>
            <organization type="academic">The Rockefeller University</organization>
            <street>1230 York Ave</street>
            <townOrCity>New York</townOrCity>
            <stateOrProvince>NY</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>10065</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0001-7250-4484</authorORCID>
            <firstName>Gregory</firstName>
            <middleName>Matthew</middleName>
            <lastName>Alushin</lastName>
            <organization type="academic">The Rockefeller University</organization>
            <street>1230 York Ave</street>
            <townOrCity>New York</townOrCity>
            <stateOrProvince>NY</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>10065</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-5056-4547">Mei L</author>
            <author authorORCID="0000-0001-7250-4484">Alushin GM</author>
        </authorsList>
        <datasetSize units="TB">2.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-10654</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-20843</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-5056-4547" order="1">Mei L</author>
                    <author authorORCID="0000-0003-4510-8296" order="2">Espinosa de Los Reyes S</author>
                    <author authorORCID="0000-0002-2501-9280" order="3">Reynolds MJ</author>
                    <author order="4">Leicher R</author>
                    <author authorORCID="0000-0003-4238-7066" order="5">Liu S</author>
                    <author authorORCID="0000-0001-7250-4484" order="6">Alushin GM</author>
                    <title>Molecular mechanism for direct actin force-sensing by α-catenin</title>
                    <journal>eLife</journal>
                    <journalAbbreviation>Elife</journalAbbreviation>
                    <country>United States</country>
                    <issue>9</issue>
                    <volume>9</volume>
                    <year>2020</year>
                    <language>English</language>
                    <externalReferences type="doi">10.7554/elife.62514</externalReferences>
                    <externalReferences type="pubmed">32969337</externalReferences>
                    <details>Each raw micrograph contains 40 movie frames. A micrograph accumulates 6 electron per pixel per second. Total exposure time is 10 seconds. Calibrated pixel size is 1.03 angstrom.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>unaligned multi-frame micrographs of alpha-catenin ABD-actin complex</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>7317</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>7420</imageWidth>
            <pixelWidth>0.515</pixelWidth>
            <imageHeight>7676</imageHeight>
            <pixelHeight>0.515</pixelHeight>
        </dimensions>
        <details>Unaligned and non-gain corrected, 40-frame movies (K2 super-resolution mode) were collected with using a Titan Krios operating at 300 kV (Rockefeller Krios I). A micrograph accumulates 6 electron per pixel per second with a defocus range of -3.5 to -1.5 um. Micrographs were auto-picked in relion. Gain reference is data/deposition_1116/reference_fliprot90.mrc</details>
        <segmentationList/>
        <micrographsFilePattern>data/deposition_1116/acat_actin_*.tif</micrographsFilePattern>
        <pickedParticlesFilePattern>data/deposition_1116/acat_picked_particles.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/deposition_1116/</pickedParticlesDirectory>
    </imageSet>
</entry>
