<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10642" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2020-10-13</depositionDate>
            <releaseDate>2021-06-11</releaseDate>
            <updateDate>2022-02-01</updateDate>
        </keyDates>
        <title>Cas6-reverse transcriptase-Cas1—Cas2 CRISPR integrase complex</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-6768-3406</authorORCID>
            <firstName>Stephen</firstName>
            <lastName>Brohawn</lastName>
            <organization type="academic">Department of Molecular and Cell Biology &amp; Helen Wills Neuroscience Institute, University of California, Berkeley</organization>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94720</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-9161-999X</authorORCID>
            <firstName>Jennifer</firstName>
            <lastName>Doudna</lastName>
            <organization type="academic">Department of Molecular and Cell Biology, University of California, Berkeley</organization>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94720</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-6768-3406</authorORCID>
            <firstName>Stephen</firstName>
            <lastName>Brohawn</lastName>
            <organization type="academic">Department of Molecular and Cell Biology &amp; Helen Wills Neuroscience Institute, University of California, Berkeley</organization>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94720</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-1344-0780">Hoel CM</author>
            <author authorORCID="0000-0001-6646-4279">Wang JY</author>
            <author authorORCID="0000-0001-9161-999X">Doudna JA</author>
            <author authorORCID="0000-0001-6768-3406">Brohawn SG</author>
        </authorsList>
        <datasetSize units="TB">2.2</datasetSize>
        <entryDOI>10.6019/EMPIAR-10642</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-22855</emdbEntry>
            <emdbEntry>EMD-22856</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0001-6646-4279" order="1">Wang JY</author>
                    <author authorORCID="0000-0002-1344-0780" order="2">Hoel CM</author>
                    <author authorORCID="0000-0002-3120-3201" order="3">Al-Shayeb B</author>
                    <author authorORCID="0000-0001-8203-8771" order="4">Banfield JF</author>
                    <author authorORCID="0000-0001-6768-3406" order="5">Brohawn SG</author>
                    <author authorORCID="0000-0001-9161-999X" order="6">Doudna JA</author>
                    <title>Structural coordination between active sites of a Cas6-reverse transcriptase-Cas1—Cas2 CRISPR integrase complex</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <year>2020</year>
                    <externalReferences type="doi">10.1101/2020.10.18.344481</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame movies of a Cas6-reverse transcriptase-Cas1—Cas2 CRISPR integrase complex</name>
        <directory>/data/Frames</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3330</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>50</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.5685</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.5685</pixelHeight>
        </dimensions>
        <details>Unaligned micrograph movies along with gain and defects files. Also included is particle stack and star file for EMD-22855.</details>
        <segmentationList/>
        <micrographsFilePattern>data/Frames/19Oct14_rtCas1-halfsite2_***_****.tif</micrographsFilePattern>
        <pickedParticlesFilePattern>data/final-particle-stack/final-particles.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/final-particle-stack/final-particles.mrcs</pickedParticlesDirectory>
    </imageSet>
</entry>
