REL
2020-12-15
2021-04-14
2021-04-14
Human NLRP1-DPP9-VbP complex
0000-0002-7281-8579
Hao
Wu
HARVARD MEDICAL SCHOOL
Boston
MA
United States
02115
0000-0002-7281-8579
Hao
Wu
HARVARD MEDICAL SCHOOL
Boston
MA
United States
02115
Hollingsworth LR
Sharif H
Wu H
1.2
10.6019/EMPIAR-10595
EMDB
EMD-22075
Hollingsworth LR
Sharif H
Griswold AR
Fontana P
Mintseris J
Dagbay KB
Paulo JA
Gygi SP
Bachovchin DA
Wu H
DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation
Nature
Nature
United States
2021
English
10.1038/s41586-021-03350-4
33731932
Unaligned multi frame micographs of NLRP1-DPP9-VbP-noTILT
/data/NLRP1_DPP9_VbP_Raw_Movies_noTILT
micrographs - focal pairs - unprocessed
TIFF
TIFF
3553
50
BIT
variable
0.825
variable
0.825
The defocus range between −0.8 to −2.2 μm and to record two shots for each of the four holes per stage movement through image shift. All movies were exposed with a total dose of 52.0 e-/Å2 for 2.0 s fractionated over 50 frames.
Unaligned multi frame micographs of NLRP1-DPP9-VbP-TILT
/data/NLRP1_DPP9_VbP_Raw_Movies_TILT
micrographs - multiframe
TIFF
TIFF
1954
43
BIT
variable
0.825
variable
0.825
An ideal tilt angle of 37° was calculated from this processed dataset using cryoEF. The defocus range between −1.5 to −3.0 μm with two shots recorded for each hole per stage movement through image shift. All movies were
exposed with a total dose of 65.0 e-/Å2 for 2.4 s fractionated over 43 frames