Single-particle Cryo-EM of the endogenous human BAF complex alone

REL 2020-10-15 2020-12-22 2020-12-22 Single-particle Cryo-EM of the endogenous human BAF complex alone 0000-0003-2606-2835 Thomas Walz Laboratory of Molecular Electron Microscopy, The Rockefeller University New York New York United States 10065 0000-0002-4058-5985 Cigall Kadoch Department of Pediatric Oncology, Dana-Faber Cancer Institute Boston Massachusetts United States 02215 Suzuki H Mashtalir N Kadoch C Walz T 258.1 10.6019/EMPIAR-10590 EMDB EMD-22476 Mashtalir N Suzuki H Farrell DP Sankar A Luo J Filipovski M D'Avino AR St Pierre R Valencia AM Onikubo T Roeder RG Han Y He Y Ranish JA DiMaio F Walz T Kadoch C A Structural Model of the Endogenous Human BAF Complex Informs Disease Mechanisms Cell Cell 3 183 802 817 2020 English 10.1016/j.cell.2020.09.051 33053319 Motion-corrected micrographs of the endogenous human BAF complex on thin-layer carbon grids /data/micrographs micrographs - single frame MRC MRC 4865 1 32 BIT FLOAT 3710 1.5 3838 1.5

Motion-corrected averages of movies collected at an accumulated dose of 66.7 e-/A^2 across 50 frames over a 15 s exposure, recorded on Talos Arctica (FEI) with a K2 Summit direct detector (Gatan) in super-resolution counting mode. Motion correction was performed using MotionCor2 with dose weighting, 2x binning, and 5x5 patches.