REL
2020-11-22
2020-12-04
2020-12-04
Single-particle Cryo-EM of the Thermococcus gammatolerans McrBC complex
0000-0002-5733-7275
Joshua
S
Chappie
Department of Molecular Medicine, Cornell University
Ithaca
New York
United States
14853
0000-0003-2606-2835
Thomas
Walz
Laboratory of Molecular Electron Microscopy, The Rockefeller University
New York
New York
United States
10065
Niu Y
Suzuki H
Hosford CJ
Walz T
Chappie JS
883.3
10.6019/EMPIAR-10583
EMDB
EMD-20866
Niu Y
Suzuki H
Hosford CJ
Walz T
Chappie JS
Structural asymmetry governs the assembly and GTPase activity of McrBC restriction complexes
Nature communications
Nat Commun
1
11
2020
English
10.1038/s41467-020-19735-4
33219217
Movies were collected at 300 kV in super-resolution mode. Total dose: 80 e/A^2 on specimen. Exposure of 10 seconds were dose-fractionated into 40 frames. The gain reference must be rotated 90 degrees and flipped upside down.
Unaligned multi-frame micrographs of the Thermococcus gammatolerans McrBC complex
/data
micrographs - multiframe
TIFF
TIFF
2178
40
UNSIGNED BYTE
7420
0.5
7676
0.5
Gain reference is included as reference.mrc in the same folder with the movie files. The gain reference must be rotated 90 degrees and flipped upside down. Dose per frame: 2 e/A^2