REL
2020-07-22
2020-11-13
2020-11-13
Single particle cryoEM of Streptococcus thermophilus SHP pheromone receptor Rgg3 in complex with SHP3
0000-0002-3194-9863
Vasileios
I
Petrou
Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers University
225 Warren Street
Newark
NJ
United States
07103
0000-0002-3194-9863
Vasileios
I
Petrou
Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers University
225 Warren Street
Newark
NJ
United States
07103
0000-0002-7039-4469
Matthew
B
Neiditch
Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers University
225 Warren Street
Newark
NJ
United States
07103
Petrou VI
Capodagli GC
Kaelber JT
Neiditch MB
3.0
10.6019/EMPIAR-10535
EMDB
EMD-22341
Capodagli GC
Tylor KM
Kaelber JT
Petrou VI
Federle MJ
Neiditch MB
Structure-function studies of Rgg binding to pheromones and target promoters reveal a model of transcription factor interplay
Proceedings of the National Academy of Sciences of the United States of America
Proc. Natl. Acad. Sci. U.S.A.
United States
39
117
24494
24502
2020
English
10.1073/pnas.2008427117
32907945
Unaligned multi-frame micrograph movies consisted of 40 frames per movie with an accumulated exposure of 47.13 electrons per angstrom squared. The movies are gain corrected.
Unaligned multi-frame micrographs of Rgg3-SHP3 complex
/data/Rgg_SHP3_2019
micrographs - multiframe
MRC
MRC
1464
40
1
40
32 BIT FLOAT
3838
1.038
3710
1.038
The dataset contains unaligned gain-corrected micrograph movies in mrc format. Cryo-EM data were collected at the Rutgers New Jersey CryoEM/CryoET Core Facility using a 200 kV Talos Arctica (FEI/ThermoFisher) electron microscope equipped with a K2 Summit direct electron detector (Gatan) and a GIF Quantum energy filter (Gatan) with slit width of 20 eV. Data were collected automatically in counting mode using EPU (FEI/ThermoFisher), a nominal magnification of 130,000X, a calibrated pixel size of 1.038 Å per pixel, and a dose rate of 6.347 electrons/pixel/s. Movies were recorded at 200 ms/frame for 8 s (40 frames total), resulting in a total radiation dose of 47.13 electrons/Å2. Defocus range was 0.5 to 3.5 μm. A total of 1,464 micrographs were recorded from two grids over two days. Micrographs were gain-normalized and defect corrected.
data/Rgg_SHP3_2019/FoilHole_*_Data_*.mrc