REL 2020-05-14 2020-11-11 2025-12-19 Single-Particle CryoEM of Influenza Hemagglutinin (HA) Trimer Vitrified Using Back-it-up 0000-0001-6656-6320 Yong Zi Tan The Hospital for Sick Children 686 Bay Street Toronto Ontario Canada M5G0A4 0000-0003-0566-2209 John L Rubinstein The Hospital for Sick Children 686 Bay Street Toronto Ontario Canada M5G0A4 Tan YZ Rubinstein JL 1.2 10.6019/EMPIAR-10532 EMDB molecule EMD-21954 Tan YZ Rubinstein JL Through-grid wicking enables high-speed cryoEM specimen preparation Acta crystallographica. Section D, Structural biology Acta Crystallogr D Struct Biol Pt 11 76 1092 1103 2020 English 10.1107/s2059798320012474 33135680 Unaligned Falcon IV movies /data/01_Raw_Frames micrographs - multiframe MRCS MRCS 1556 30 1 30 SIGNED 16 BIT INTEGER 4096 1.03 4096 1.03
1,556 movies were acquired from one grid with hemagglutinin prepared with the BIU device. The calibrated pixel size was 1.03 Å. Movies were collected for 9 sec with 29 exposure fractions, a camera exposure rate of ~5 e–/pix/sec, and a total specimen exposure of ~40 e-/Å2. No objective aperture was used. Note: Due to software issues, the last frames in these raw movies have errors. Please exclude the last frame (frame 30) when doing any alignments.
data/02_Aligned_Micrographs/motioncorrected/*doseweighted.mrc data/03_Refined_Particles/P30_J363_particles/P30_J363_particles_exported.cs data/03_Refined_Particles/P30_J363_particles/ Aligned micrographs /data/02_Aligned_Micrographs/motioncorrected micrographs - single frame MRC MRC 1556 1 32 BIT FLOAT 4096 1.03 4096 1.03
Micrographs were aligned using cryoSPARC patch motion correction.
data/02_Aligned_Micrographs/motioncorrected/*doseweighted.mrc data/03_Refined_Particles/P30_J363_particles/P30_J363_particles_exported.cs data/03_Refined_Particles/P30_J363_particles/ Final Refined Particles with Euler Angles and Shifts /data/03_Refined_Particles/P30_J363_particles/J342/localmotioncorrected picked particles - single frame - processed MRC MRC 128305 1 32 BIT FLOAT 256 1.03 256 1.03
Final refined particles from cryoSPARC with Euler angles and shifts.
data/02_Aligned_Micrographs/motioncorrected/*doseweighted.mrc data/03_Refined_Particles/P30_J363_particles/P30_J363_particles_exported.cs data/03_Refined_Particles/P30_J363_particles/