REL 2020-08-27 2021-10-01 2022-01-31 0000-0001-8943-1330 Thomas Gerard Laughlin Department of Molecular & Cell Biology, Division of Biochemistry, Biophysics and Structural Biology, University of California-Berkeley Berkeley CA United States 94702 0000-0003-0042-2257 David Savage Department of Molecular & Cell Biology, Division of Biochemistry, Biophysics and Structural Biology, University of California-Berkeley Berkeley CA United States 94702 Laughlin TG Shams A Savage DF 1.5 10.6019/EMPIAR-10508 EMDB EMD-22518 Shams A Higgins SA Fellmann C Laughlin TG Oakes BL Lew R Kim S Lukarska M Arnold M Staahl BT Doudna JA Savage DF Nature communications Nat Commun 1 12 2021 English 10.1038/s41467-021-25992-8 34580310 Unaligned multi-frame, non-gain-corrected frames in LZW-TIFF of SpCas9 delta4CE Ternary Complex /data/frames micrographs - multiframe MRC TIFF 2570 60 UNSIGNED BYTE 11520 0.45 8184 0.45

LZW-TIFF, non-gain-corrected, super-resolution K3 multi-frame movies containing 60 frames with cumulative, uniform exposure of 60 electrons per square Angstrom. Gain-reference provided in frame directory as dm4 format. Final particle positions and orientations provided as run_data.star from RELION-3.1b. Data collected using SerialEM with a 3x3 image-shift pattern and beam tilt compensation, but 9th position was systematically poor and removed yielding 8 image-shift groups as marked by the trailing 4 digit-identifier of each movie file.

data/frames/*.tif data/run_data.star