<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10507" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2020-08-27</depositionDate>
            <releaseDate>2020-10-16</releaseDate>
            <updateDate>2020-10-16</updateDate>
        </keyDates>
        <title>Structure of human Frizzled5 by fiducial-assisted cryo-EM</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-9273-0278</authorORCID>
            <firstName>K Christopher</firstName>
            <lastName>Garcia</lastName>
            <organization type="academic">Howard Hughes Medical Institute, Depts. of Molecular and Cellular Physiology, and Structural Biology, Stanford University School of Medicine</organization>
            <street>Beckman B171B, 279 Campus Drive</street>
            <townOrCity>Stanford</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94305</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-9273-0278</authorORCID>
            <firstName>K Christopher</firstName>
            <lastName>Garcia</lastName>
            <organization type="academic">Howard Hughes Medical Institute, Depts. of Molecular and Cellular Physiology, and Structural Biology, Stanford University School of Medicine</organization>
            <street>Beckman B171B, 279 Campus Drive</street>
            <townOrCity>Stanford</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94305</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-3617-7145">Tsutsumi N</author>
            <author authorORCID="0000-0003-3693-7009">Gati C</author>
            <author authorORCID="0000-0001-9273-0278">Garcia KC</author>
        </authorsList>
        <datasetSize units="TB">6.0</datasetSize>
        <entryDOI>10.6019/EMPIAR-10507</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-21927</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-3617-7145" order="1">Tsutsumi N</author>
                    <author authorORCID="0000-0001-5447-4496" order="2">Mukherjee S</author>
                    <author order="3">Waghray D</author>
                    <author authorORCID="0000-0002-3307-2559" order="4">Janda CY</author>
                    <author authorORCID="0000-0002-3675-5136" order="5">Jude KM</author>
                    <author authorORCID="0000-0003-0738-6041" order="6">Miao Y</author>
                    <author order="7">Burg JS</author>
                    <author authorORCID="0000-0003-4301-7446" order="8">Aduri NG</author>
                    <author authorORCID="0000-0003-3174-9359" order="9">Kossiakoff AA</author>
                    <author authorORCID="0000-0003-3693-7009" order="10">Gati C</author>
                    <author authorORCID="0000-0001-9273-0278" order="11">Garcia KC</author>
                    <editor authorORCID="0000-0001-6965-0851" order="1">Boudker O</editor>
                    <editor authorORCID="0000-0003-1898-8099" order="2">Zeng YA</editor>
                    <title>Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling</title>
                    <journal>eLife</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United Kingdom</country>
                    <issue>9</issue>
                    <year>2020</year>
                    <language>English</language>
                    <externalReferences type="doi">10.7554/eLife.58464</externalReferences>
                    <externalReferences type="pubmed">32762848</externalReferences>
                    <details>Unaligned dark-subtracted TIFF movies with a gain reference for the 3D reconstruction of EMD-21927.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned dark-subtracted TIFF movies with a gain reference for the 3D reconstruction of EMD-21927.</name>
        <directory>/data/20190906_Janelia_Krios2_hFz5ICL3BRIL</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>10922</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.539</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.539</pixelHeight>
        </dimensions>
        <details>Set defocus range: -0.8 um to -2.0 um
Exposure rate at specimen: 9.5 e/pix/s
Total exposure: 50 e over 6.125 s (0.1225 s/frame)
Exposure rate at camera: 7.5~8.6 e/pix/s (6.45~ e/A2/s, 0.8~ e/A2/frame)</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
