Integrative imaging reveals SARS-CoV-2 induced reshaping of subcellular morphology

REL 2020-06-24 2020-12-11 2021-10-25 Integrative imaging reveals SARS-CoV-2 induced reshaping of subcellular morphology 0000-0001-5601-9307 Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, Heidelberg University Heidelberg Germany 69120 0000-0001-5601-9307 Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, Heidelberg University Heidelberg Germany 69120 Yannick Schwab Cell Biology and Biophysics Unit, EMBL Heidelberg Germany 69117 Cortese M Lee JY Cerikan B Neufeldt CJ Oorschot VMJ Köhrer S Hennies J Schieber NL Ronchi P Mizzon G Romero-Brey I Santarella-Mellwig R Schorb M Boermel M Mocaer K Beckwith MS Templin RM Gross V Pape C Tischer C Frankish J Horvat NK Laketa V Stanifer M Boulant S Ruggieri A Chatel-Chaix L Schwab Y Bartenschlager R 1.1 10.6019/EMPIAR-10490 IHM EMD-12940 Cortese M Lee JY Cerikan B Neufeldt CJ Oorschot VMJ Köhrer S Hennies J Schieber NL Ronchi P Mizzon G Romero-Brey I Santarella-Mellwig R Schorb M Boermel M Mocaer K Beckwith MS Templin RM Gross V Pape C Tischer C Frankish J Horvat NK Laketa V Stanifer M Boulant S Ruggieri A Chatel-Chaix L Schwab Y Bartenschlager R Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies Cell host & microbe Cell Host Microbe 2020 English 10.1016/j.chom.2020.11.003 33245857 MOI0.5-24h-Dual axis tilt series /data/Tomography/Calu3_MOI0.5_24h_H2/raw_tilt tilt series MRC MRC 202 121 UNSIGNED 16 BIT INTEGER 2024 15.578 2024 15.578

Condition: MOI = 0.5, 24h post infection; Dual axis tilt series, both axes as individual *.st MRC files

MOI5-12h-Dual axis tilt series /data/Tomography/Calu3_MOI5_12h_E3/raw_tilt tilt series MRC MRC 178 121 UNSIGNED 16 BIT INTEGER 2024 15.578 2024 15.578

Condition: MOI = 5, 12h post infection; Dual axis tilt series, both axes as individual *.st MRC files

MOI5-24h-Dual axis tilt series /data/Tomography/Calu3_MOI5_24h_C2/raw_tilt tilt series MRC MRC 152 121 UNSIGNED 16 BIT INTEGER 2024 15.578 2024 15.578

Condition: MOI = 5, 24h post infection; Dual axis tilt series, both axes as individual *.st MRC files

MOI5-6h-Dual axis tilt series /data/Tomography/Calu_MOI5_6h_K2/raw_tilt tilt series MRC MRC 14 121 UNSIGNED 16 BIT INTEGER 2024 15.578 2024 15.578

Condition: MOI = 5, 6h post infection; Dual axis tilt series, both axes as individual *.st MRC files

MOI0.5-24h-Reconstructed tomograms /data/Tomography/Calu3_MOI0.5_24h_H2/tomos reconstructed volumes XML BIG DATA VIEWER HDF5 97 1 UNSIGNED BYTE variable 15.578 variable 15.578

Condition: MOI = 0.5, 24h post infection; Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.

MOI5-12h-Reconstructed tomograms /data/Tomography/Calu3_MOI5_12h_E3/tomos reconstructed volumes XML BIG DATA VIEWER HDF5 85 1 UNSIGNED BYTE variable 15.578 variable 15.578

Condition: MOI = 5, 12h post infection; Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.

MOI5-24h-Reconstructed tomograms /data/Tomography/Calu3_MOI5_24h_C2/tomos reconstructed volumes XML BIG DATA VIEWER HDF5 56 1 UNSIGNED BYTE variable 15.578 variable 15.578

Condition: MOI = 5, 24h post infection; Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.

MOI5-6h-Reconstructed tomograms /data/Tomography/Calu_MOI5_6h_K2/tomos reconstructed volumes XML BIG DATA VIEWER HDF5 7 1 UNSIGNED BYTE variable 15.578 variable 15.578

Condition: MOI = 5, 6h post infection; Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.

S4 Area 2 FIB-SEM Volume 8nm isotropic /data/FIB-SEM/S4_area2 micrographs - single frame TIFF TIFF 4824 1 UNSIGNED BYTE 3864 80.0 3144 80.0

S4 Area 2 FIB-SEM Volume 8nm isotropic voxel size, aligned

data/FIB-SEM/S4_area2/*.tif S4 Area 3 FIB-SEM Volume 8nm isotropic /data/FIB-SEM/S4_area3 micrographs - single frame TIFF TIFF 4413 1 UNSIGNED BYTE 5632 80.0 3648 80.0

S4 Area 3 FIB-SEM Volume 8nm isotropic voxel size, aligned

data/FIB-SEM/S4_area3/*.tif S4 Cell 1 FIB-SEM Volume 5nm isotropic /data/FIB-SEM/S4_cell1_5nm micrographs - single frame TIFF TIFF 11199 1 UNSIGNED BYTE 7512 50.0 3864 50.0

S4 Cell 1 FIB-SEM Volume 5nm isotropic voxel size, aligned

data/FIB-SEM/S4_cell1_5nm/*.tif S5 Mock Cell 1 FIB-SEM Volume 8nm isotropic /data/FIB-SEM/S5_mock_cell1_2 micrographs - single frame TIFF TIFF 2574 1 UNSIGNED BYTE 6624 80.0 4236 80.0

S5 Mock/Control Cell 1 FIB-SEM Volume 8nm isotropic voxel size, aligned

data/FIB-SEM/S5_mock_cell1_2/*.tif Mock cells - Dual axis tilt series /data/Tomography/Mock/raw_tilts tilt series MRC MRC 26 121 UNSIGNED 16 BIT INTEGER 2024 15.578 2024 15.578

Condition: Mock; Dual axis tilt series, both axes as individual *.st MRC files

Mock cells-Reconstructed tomograms /data/Tomography/Mock/tomos reconstructed volumes XML BIG DATA VIEWER HDF5 13 1 UNSIGNED BYTE variable 15.578 variable 15.578

Condition: Mock; Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.