REL 2020-07-04 2020-09-02 2021-05-07 0000-0002-6247-4063 Bjoern O. Forsberg Stockholm University, Science for Life Laboratory Tomtebodavägen 23A SOLNA Sweden 17165 0000-0002-2734-2794 Erik Lindahl Stockholm University, Science for Life Laboratory Tomtebodavägen 23A SOLNA SOLNA Sweden 17165 Forsberg BO Aibara S Howard RJ Mortezaei N Lindahl E 305.5 10.6019/EMPIAR-10489 EMDB EMD-11266 EMD-11267 EMD-11268 Forsberg BO Aibara S Howard RJ Mortezaei N Lindahl E Nature communications Nat Commun 1 11 2020 English 10.1038/s41467-020-18401-z 32938938 Aligned and dose-weighted micrographs of native N. crassa Pyruvate dehydrogenase /data/MG micrographs - single frame MRC MRC 4887 1 32 BIT FLOAT 4096 1.25 4096 1.25

All motion-corrected, dose-weighted and gain-corrected micrographs are contained in the directory "MG". No extracted particles are included. Instead, particles can be re-extracted using the .star files. Estimated CTF values per-micrograph is found in the file micrographs_ctf.star, which should be used to re-extract particles defined in any particles.star-file. The latter files contain refined positions and angle assignments. all_particles.star contain all particles considered to be non-junk, PDC-particles. Angle assignments are based on classification of these particles into class X, class Y, and class N, as denoted in the paper "Arrangement and symmetry of the fungal E3BP-containing core of the Pyruvate Dehydrogenase Complex". Two separate files contain the re-refined angles of class X and Yi respectively, based on gold-standard refinement using relion.

data/MG/*mrc data/all_particles.star