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        <keyDates>
            <depositionDate>2020-06-24</depositionDate>
            <releaseDate>2020-07-21</releaseDate>
            <updateDate>2020-07-21</updateDate>
        </keyDates>
        <title>Single particle cryo EM dataset of SARS-CoV-2 Spike protein in uncleavable form, which generated a 2.6Å reconstruction of the closed trimer.</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-6748-9339</authorORCID>
            <firstName>Donald</firstName>
            <lastName>Benton</lastName>
            <organization type="academic">Francis Crick Institute</organization>
            <street>1 Midland Road</street>
            <townOrCity>London</townOrCity>
            <stateOrProvince>London</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>NW1 1AT</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-5331-639X</authorORCID>
            <firstName>Steven</firstName>
            <lastName>Gamblin</lastName>
            <organization type="academic">Francis Crick Institute</organization>
            <street>1 Midland Road</street>
            <townOrCity>London</townOrCity>
            <stateOrProvince>London</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>NW1 1AT</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-0387-2862</authorORCID>
            <firstName>Peter</firstName>
            <lastName>Rosenthal</lastName>
            <organization type="academic">Francis Crick Institute</organization>
            <street>1 Midland Road</street>
            <townOrCity>London</townOrCity>
            <stateOrProvince>London</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>NW1 1AT</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-6680-5587">Wrobel AG</author>
            <author authorORCID="0000-0001-6748-9339">Benton DJ</author>
            <author authorORCID="0000-0002-3791-2447">Nans A</author>
            <author authorORCID="0000-0002-0387-2862">Rosenthal PB</author>
            <author authorORCID="0000-0001-5331-639X">Gamblin SJ</author>
        </authorsList>
        <datasetSize units="TB">8.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-10469</entryDOI>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-11203</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
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                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-6680-5587" order="1">Wrobel AG</author>
                    <author authorORCID="0000-0001-6748-9339" order="2">Benton DJ</author>
                    <author order="3">Xu P</author>
                    <author order="4">Roustan C</author>
                    <author order="5">Martin SR</author>
                    <author authorORCID="0000-0002-0387-2862" order="6">Rosenthal PB</author>
                    <author authorORCID="0000-0002-8519-9404" order="7">Skehel JJ</author>
                    <author authorORCID="0000-0001-5331-639X" order="8">Gamblin SJ</author>
                    <title>SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects</title>
                    <journal>Nature Structural and Molecular Biology</journal>
                    <journalAbbreviation>Nat. Struct. Mol. Biol.</journalAbbreviation>
                    <country>United Kingdom</country>
                    <year>2020</year>
                    <externalReferences type="doi">10.1038/s41594-020-0468-7</externalReferences>
                    <externalReferences type="pubmed">32647346</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned Movies of uncleavable SARS-CoV-2 Spike protein ectodomain</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>9210</numImagesOrTiltSeries>
        <framesPerImage>30</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>1.087</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>1.087</pixelHeight>
        </dimensions>
        <details>Movies collected of uncleavable SARS-CoV-2 S protein. Movies collected on a Titan Krios at 300kV on a Falcon 3 detector in electron counting mode. Movies are 30 frames with 1.13 e/Å^2/frame with a total dose of 33.9 e/Å^2.</details>
        <segmentationList/>
        <micrographsFilePattern>data/Images-Disc1/GridSquare_*/Data/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
